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BD OptiBuild™ BV605 Rat Anti-Mouse IgA
品牌： BD Pharmingen
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对比
咨询反应种属：
Mouse (Tested in Development)
来源宿主：
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
产品介绍
产品介绍
产品信息
荧光素标记
抗原名称
IgA
宿主
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
免疫原
Pooled mouse IgA
简单描述
The C10-1 antibody reacts specifically with mouse IgA of
Igh-C[a]
and
Igh-C[b]
haplotypes. It does not react with other Ig isotypes. Detection of surface immunoglobulin on B lymphoma cells has been demonstrated with C10-1 mAb.
This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.
商品描述
C10-1
The C10-1 antibody reacts specifically with mouse IgA of
Igh-C[a]
and
Igh-C[b]
haplotypes. It does not react with other Ig isotypes. Detection of surface immunoglobulin on B lymphoma cells has been demonstrated with C10-1 mAb.
This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set. BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm. Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers. BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.
同种型
Rat LOU, also known as Louvain, LOU/C, LOU/M IgG1, κ
克隆号
克隆 C10-1 (RUO)
浓度
0.2 mg/ml
产品详情
BV605
The BD Horizon Brilliant Violet™ 605 (BV605) dye is part of the BD Horizon Brilliant Violet™ family of dyes. This tandem fluorochrome is comprised of a BV421 donor with an excitation maximum (Ex Max) of 407-nm and an acceptor dye with an emission maximum (Em Max) at 605-nm. BV605, driven by BD innovation, is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 610-nm (e.g., a 610/20-nm bandpass filter). The acceptor dye can be excited by the yellow-green (561-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BV605
Violet 405 nm
407 nm
605 nm
应用
实验应用
Flow cytometry (Qualified)
反应种属
Mouse (Tested in Development)
目标/特异性
IgA
背景
别名
IgA; Igh-2; Igha; Immunoglobulin heavy constant alpha
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(2)
1. Jaffar Z, Ferrini ME, Herritt LA, Roberts K. Cutting edge: lung mucosal Th17-mediated responses induce polymeric Ig receptor expression by the airway epithelium and elevate secretory IgA levels.. J Immunol. 2009; 182(8):4507-11. (Clone-specific: Dot Blot).
2. Lazarus NH, Kunkel EJ, Johnston B, Wilson E, Youngman KR, Butcher EC. A common mucosal chemokine (mucosae-associated epithelial chemokine/CCL28) selectively attracts IgA plasmablasts.. J Immunol. 2003; 170(7):3799-805. (Clone-specific: Flow cytometry).
参考图片
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