全部商品分类
用小程序，查商品更便捷
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro184 of mouse CD81 protein.
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
| Immunofluorescence (Immunocytochemistry) | 1:200 - 1:400 |
| Flow Cytometry (Live) | 1:50 |
Specificity/Sensitivity
Species Reactivity:
Mouse
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #79273.
参考图片
Flow cytometric analysis of live EL4 cells (blue, negative) and A20 cells (green, positive) using CD81 (D5O2Q) Rabbit mAb (solid lines) or concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (dashed line). Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4412 was used as a secondary antibody.
Western blot analysis of extracts from various cell lines using CD81 (D5O2Q) Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).
Western blot analysis of extracts from 293T cells, untransfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length mouse CD81 (mCD81-Myc/DDK, +), using CD81 (D5O2Q) Rabbit mAb.
Immunoprecipitation of CD81 from extracts of C2C12 cells. Lane 1 is 10% input, Lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is CD81 (D5O2Q) Rabbit mAb. Western blot analysis was performed using CD81 (D5O2Q) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded CT26.WT syngeneic tumor using CD81 (D5O2Q) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse testis using CD81 (D5O2Q) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse lung using CD81 (D5O2Q) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse cerebellum using CD81 (D5O2Q) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse striatum using CD81 (D5O2Q) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse brown fat using CD81 (D5O2Q) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse colon using CD81 (D5O2Q) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse heart using CD81 (D5O2Q) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse forestomach using CD81 (D5O2Q) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse liver using CD81 (D5O2Q) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).
Immunohistochemical analysis of paraffin-embedded C2C12 cell pellet (left, positive) or Ba/F3 cell pellet (right, negative) using CD81 (D5O2Q) Rabbit mAb.
Confocal immunofluorescent analysis of C2C12 (positive, left) and BaF3 (negative, right) cells using CD81 (D5O2Q) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).