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BD Horizon™ BB515 Mouse Anti-Human CD86

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BD Horizon™ BB515 Mouse Anti-Human CD86

品牌： BD Pharmingen

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反应种属：

Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)

来源宿主：

Mouse BALB/c IgG1, κ

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产品介绍

产品信息

耦联标记

BB515

抗原名称

CD86 (B7-2)

宿主

Mouse BALB/c IgG1, κ

免疫原

Human HBL-1 Cell Line

简单描述

The 2331 (FUN-1) monoclonal antibody specifically recognizes a 75 kDa transmembrane cell surface protein, CD86 (B70/B7-2), expressed primarily on monocytes, dendritic cells and activated B cells. Competitive binding assays demonstrate that, while both 2331 (FUN-1) and IT2.2 (Anti-CD86) antibodies specifically recognize the same molecule, they react with different epitopes. CD86 is a ligand for CD28 and CTLA-4 and plays an important role in costimulation of T cells in primary immune response. The 2331 (FUN-1) antibody blocks the costimulatory activity of CD86 when tested in functional studies. The antibody was conjugated to BD Horizon BB515 which is part of the BD Horizon Brilliant™ Blue family of dyes. With an Ex Max near 490 nm and an Em Max near 515 nm, BD Horizon BB515 can be excited by the blue laser (488 nm) laser and detected with a 530/30 nm filter. This dye has been exclusively developed by BD Biosciences and is up to seven times brighter than FITC with less spillover into the PE channel. Due to similar excitation and emission properties, BB515, FITC, and Alexa Fluor® 488 cannot be used simultaneously. It is not recommended to use BB515 in cocktails that include Streptavidin conjugates as it may cause high background.

商品描述

2331 (FUN-1) The 2331 (FUN-1) monoclonal antibody specifically recognizes a 75 kDa transmembrane cell surface protein, CD86 (B70/B7-2), expressed primarily on monocytes, dendritic cells and activated B cells. Competitive binding assays demonstrate that, while both 2331 (FUN-1) and IT2.2 (Anti-CD86) antibodies specifically recognize the same molecule, they react with different epitopes. CD86 is a ligand for CD28 and CTLA-4 and plays an important role in costimulation of T cells in primary immune response. The 2331 (FUN-1) antibody blocks the costimulatory activity of CD86 when tested in functional studies. The antibody was conjugated to BD Horizon BB515 which is part of the BD Horizon Brilliant™ Blue family of dyes. With an Ex Max near 490 nm and an Em Max near 515 nm, BD Horizon BB515 can be excited by the blue laser (488 nm) laser and detected with a 530/30 nm filter. This dye has been exclusively developed by BD Biosciences and is up to seven times brighter than FITC with less spillover into the PE channel. Due to similar excitation and emission properties, BB515, FITC, and Alexa Fluor® 488 cannot be used simultaneously. It is not recommended to use BB515 in cocktails that include Streptavidin conjugates as it may cause high background.

同种型

Mouse BALB/c IgG1, κ

克隆号

克隆 2331 (FUN-1) (RUO)

产品详情

BB515

The BD Horizon Brilliant™ Blue 515 (BB515) dye is part of the BD Horizon Brilliant™ Blue family of dyes. This dye is a polymer fluorochrome with an excitation maximum (Ex Max) at 490-nm and an emission maximum (Em Max) of 515-nm. Driven by BD innovation, BB515 is designed to be excited by the blue laser (488-nm) and detected using an optical filter centered near 520-nm (e.g., 530/30-nm). BB515 reagents are significantly brighter than equivalent FITC or Alexa Fluor™ 488 reagents with less spillover into the PE detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式:BB515

激发源:Blue 488 nm

最多激发源:490 nm

最多发射源:515 nm

应用

实验应用

Flow cytometry (Routinely Tested)

推荐用量

5 µl

反应种属

Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)

目标/特异性

CD86 (B7-2)

背景

别名

B7.2; B7-2; B-lymphocyte activation antigen B7-2; B70; BU63; CD28LG2; LAB72

制备和贮存

存储溶液

Aqueous buffered solution containing ≤0.09% sodium azide.

保存方式

Aqueous buffered solution containing ≤0.09% sodium azide.

文献

研发参考(5) 1. Engel P, Gribben JG, Freeman GJ, et al. The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen. Blood. 1994; 84(5):1402-1407. (Clone-specific: Blocking, Enhancement, Flow cytometry, Functional assay, Inhibition). 2. Engel P, Wagner N, Tedder TF. CD86 Workshop Report. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:703-705. 3. Nozawa Y, Abe M, Wakasa H. Three mAb, FUN-1, FB1, and FB21, that recognize B-cell antigens in frozen or paraffin-embedded tissue sections. In: Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995:705-706. 4. Nozawa Y, Wachi E, Tominaga K, Abe M, Wakasa H. A novel monoclonal antibody (FUN-1) identifies an activation antigen in cells of the B-cell lineage and Reed-Sternberg cells. J Pathol. 1993; 169(3):309-315. (Immunogen: Flow cytometry, Fluorescence microscopy, Immunofluorescence, Immunohistochemistry, Immunoprecipitation). 5. Nozawa Y, Wakasa H, Abe M. Production and usefulness of monoclonal antibodies against B cells. Fukushima J Med Sci. 1999; 45(1):1-11. (Clone-specific).

数据库链接

Entrez-Gene ID

942

参考图片

Flow cytometric analysis of CD86 expression on Daudi cells - Staining comparisons between BD Horizon™ BB515- and FITC-conjugated antibodies. Human Daudi B lymphoma cells (ATCC CCL-213) were stained with either BD Horizon BB515 Mouse IgG1, κ Isotype Control (Cat. No. 564416; dashed line histogram) or BD Horizon BB515 Mouse Anti-Human CD86 antibody (Cat. No. 564544/564545; bold solid line histograms). Alternatively, cells were stained with FITC Anti-Human CD86 antibody (Cat. No. 555657/557343/560958; thin solid line histogram). Overlaid histograms are shown to facilitate staining comparisons between: BB515 Anti-CD86 antibody versus its Ig Isotype Control (Left Panel), and BB515 Anti-CD86 antibody versus FITC Anti-CD86 antibody (Right Panel). The fluorescence histograms showing CD86 expression (or Ig Isotype control staining) were derived from gated events with the forward and side light-scatter characteristics of viable cells. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.

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货号：

564544

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