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Rabbit anti-CD86 Polyclonal Antibody
Rabbit anti-CD86 Polyclonal Antibody
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Rabbit anti-CD86 Polyclonal Antibody

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品牌： Absin
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    分子量：
    Predicted molecular weight: 31kDa
    Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
    Predicted molecular weight: 31kDa
    Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
    反应种属：
    Human;Mouse;Rat;
    Human;Mouse;Rat;
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    产品介绍
    产品信息
    耦联标记
    Unlabeled
    纯化方式
    affinity purified by Protein A
    宿主
    Rabbit
    免疫原
    KLH conjugated synthetic peptide derived from the middle of rat CD86
    同种型
    IgG
    分子量
    Predicted molecular weight: 31kDa
    Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
    克隆类别
    Polyclonal Antibody
    浓度
    1mg/mL
    应用
    实验应用
    WB: 1:500-2000, IHC-F: 1:100-500, IHC-P: 1:100-500, IF: 1:100-500, FCM: 2ug/Test
    反应种属
    Human;Mouse;Rat;
    目标/特异性
    CD86
    背景
    别名
    CD86抗体
    研究领域
    免疫学 微生物学 干细胞 细胞表面分子 糖蛋白 淋巴细胞 t-淋巴细胞 b-淋巴细胞
    翻译后修饰
    Unmodified
    无
    Expressed by activated B-lymphocytes and monocytes.
    细胞定位
    细胞膜
    制备和贮存
    存储溶液
    0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    保存方式
    Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

    参考图片

    Sample: Lane 1: HepG2(Human) Cell Lysate at 30 ug Lane 2: U937(Human) Cell Lysate at 30 ug Lane 3: Spleen (Rat) Lysate at 40 ug Lane 4: Spleen (Mouse) Lysate at 40 ug Lane 5: HL-60(Human) Cell Lysate at 30 ug Primary: Anti-CD86 at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 72-74 kDa Observed band size: 72 kDa

    Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer(0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer(normal goat serum) at 37℃ for 20 min; Incubation: Anti-CD86/B7-2 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

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    货号：
    abs120515-50ul
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    50ul
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