Rabbit anti-CD86 Polyclonal Antibody

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Rabbit anti-CD86 Polyclonal Antibody

品牌： Absin

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产品介绍

产品信息

荧光素标记

Unlabeled

纯化方式

affinity purified by Protein A

宿主

Rabbit

免疫原

KLH conjugated synthetic peptide derived from the middle of rat CD86(140-175/313)

同种型

IgG

分子量

Predicted molecular weight: 31kDa

克隆类别

Polyclonal Antibody

浓度

1mg/mL

应用

实验应用

WB: 1:500-2000, IHC-F: 1:100-500, IHC-P: 1:100-500, IF: 1:100-500, FCM: 2ug/Test

反应种属

Human, Mouse, Rat

目标/特异性

CD86

背景

别名

CD86抗体

研究领域

免疫学微生物学干细胞细胞表面分子糖蛋白淋巴细胞 t-淋巴细胞 b-淋巴细胞

翻译后修饰

Unmodified

无

Expressed by activated B-lymphocytes and monocytes.

细胞定位

细胞膜

制备和贮存

存储溶液

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存方式

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

参考图片

Sample: Lane 1: HepG2(Human) Cell Lysate at 30 ug Lane 2: U937(Human) Cell Lysate at 30 ug Lane 3: Spleen (Rat) Lysate at 40 ug Lane 4: Spleen (Mouse) Lysate at 40 ug Lane 5: HL-60(Human) Cell Lysate at 30 ug Primary: Anti-CD86 at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 72-74 kDa Observed band size: 72 kDa

Tissue/cell: rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer(0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer(normal goat serum) at 37℃ for 20 min; Incubation: Anti-CD86/B7-2 Polyclonal Antibody, Unconjugated 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

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货号：

abs120515-50ul

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