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Hu CD8 BV421 G42-8 50ug
品牌： BD Pharmingen
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咨询反应种属：
Human (Tested in Development)
来源宿主：
Mouse IgG2a, κ
产品介绍
产品使用步骤推荐 产品介绍
产品信息
荧光素标记
BV421()
抗原名称
CD8
宿主
Mouse IgG2a, κ
简单描述
The G42-8 monoclonal antibody specifically recognizes the alpha subunit (32 kDa) of CD8αα homodimer or CD8αβ heterodimer. The CD8 molecule binds to HLA class I molecules during interaction of CD8+ T cells with antigen-presenting cells or target cells. CD8 is expressed on T cytotoxic/suppressor cell populations. G42-8 stains approximately 13-48% of peripheral blood lymphocytes and 80% of thymocytes, as well as a subset of NK cells. This antibody is useful in detecting CD8+ T cells when using DimerX I reagents.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.
商品描述
G42-8
The G42-8 monoclonal antibody specifically recognizes the alpha subunit (32 kDa) of CD8αα homodimer or CD8αβ heterodimer. The CD8 molecule binds to HLA class I molecules during interaction of CD8+ T cells with antigen-presenting cells or target cells. CD8 is expressed on T cytotoxic/suppressor cell populations. G42-8 stains approximately 13-48% of peripheral blood lymphocytes and 80% of thymocytes, as well as a subset of NK cells. This antibody is useful in detecting CD8+ T cells when using DimerX I reagents.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.
同种型
Mouse IgG2a, κ
克隆号
克隆 G42-8 (RUO)
浓度
0.2 mg/ml
产品详情
BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BD化合物表
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应用
实验应用
Flow cytometry (Qualified)
反应种属
Human (Tested in Development)
目标/特异性
CD8
背景
别名
CD8α; CD8A; CD8 alpha; Leu2; MAL; T8; p32
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(3)
1. Baev DV, Peng XH, Song L, et al. Distinct homeostatic requirements of CD4+ and CD4- subsets of Valpha24-invariant natural killer T cells in humans.. Blood. 2004; 104(13):4150-6. (Clone-specific: Cell separation).
2. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
3. Shih CC, Bolton G, Sehy D, Lay G, Campbell D, Huang CM. A novel dye that facilitates three-color analysis of PBMC by flow cytometry.. Ann N Y Acad Sci. 1993; 677:389-95. (Clone-specific).
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