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BD OptiBuild™ BV421 Mouse Anti-Human CD8
品牌： BD Pharmingen
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咨询反应种属：
Human (Tested in Development)
来源宿主：
Mouse IgG2a, κ
产品介绍
产品介绍
产品信息
荧光素标记
抗原名称
CD8
宿主
Mouse IgG2a, κ
简单描述
The G42-8 monoclonal antibody specifically recognizes the alpha subunit (32 kDa) of CD8αα homodimer or CD8αβ heterodimer. The CD8 molecule binds to HLA class I molecules during interaction of CD8+ T cells with antigen-presenting cells or target cells. CD8 is expressed on T cytotoxic/suppressor cell populations. G42-8 stains approximately 13-48% of peripheral blood lymphocytes and 80% of thymocytes, as well as a subset of NK cells. This antibody is useful in detecting CD8+ T cells when using DimerX I reagents.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.
商品描述
G42-8
The G42-8 monoclonal antibody specifically recognizes the alpha subunit (32 kDa) of CD8αα homodimer or CD8αβ heterodimer. The CD8 molecule binds to HLA class I molecules during interaction of CD8+ T cells with antigen-presenting cells or target cells. CD8 is expressed on T cytotoxic/suppressor cell populations. G42-8 stains approximately 13-48% of peripheral blood lymphocytes and 80% of thymocytes, as well as a subset of NK cells. This antibody is useful in detecting CD8+ T cells when using DimerX I reagents.
The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 407-nm and Em Max at 421-nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50-nm filter). BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates.
同种型
Mouse IgG2a, κ
克隆号
克隆 G42-8 (RUO)
浓度
0.2 mg/ml
产品详情
BV421
The BD Horizon Brilliant Violet™ 421 (BV421) Dye is part of the BD Horizon Brilliant Violet™ family of dyes. This polymer-technology based dye has an excitation maximum (Ex Max) of 407-nm and an emission maximum (Em Max) at 423-nm. Driven by BD innovation, BV421 is designed to be excited by the violet laser (405-nm) and detected using an optical filter centered near 420-nm (e.g., a 431/28-nm or 450/50-nm bandpass filter). BV421 is an ideal alternative for V450 as it is approximately ten times brighter with less spillover into the BV510/V500 detector. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
BV421
Violet 405 nm
407 nm
423 nm
应用
实验应用
Flow cytometry (Qualified)
反应种属
Human (Tested in Development)
目标/特异性
CD8
背景
别名
CD8α; CD8A; CD8 alpha; Leu2; MAL; T8; p32
制备和贮存
存储溶液
Aqueous buffered solution containing ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing ≤0.09% sodium azide.
文献
文献
研发参考(3)
1. Baev DV, Peng XH, Song L, et al. Distinct homeostatic requirements of CD4+ and CD4- subsets of Valpha24-invariant natural killer T cells in humans.. Blood. 2004; 104(13):4150-6. (Clone-specific: Cell separation).
2. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
3. Shih CC, Bolton G, Sehy D, Lay G, Campbell D, Huang CM. A novel dye that facilitates three-color analysis of PBMC by flow cytometry.. Ann N Y Acad Sci. 1993; 677:389-95. (Clone-specific).
数据库链接
Entrez-Gene ID
925
参考图片
Multiparameter flow cytometric analysis using BD OptiBuild™ BV421 Mouse Anti-Human CD8 antibody (Cat. No. 743064) on human peripheral blood. Flow cytometry was performed using a BD LSRFortessa™ X-20 Flow Cytometer System.
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