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CD8alpha (D8A8Y) Rabbit mAb

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CD8alpha (D8A8Y) Rabbit mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

抗原名称

CD8alpha

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human CD8α protein.

宿主

Rabbit

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:100
Immunohistochemistry (Paraffin)	1:100 - 1:400
Flow Cytometry (Fixed/Permeabilized)	1:50 - 1:200

同种型

Rabbit IgG

分子量

研究领域

癌症,纤维化,免疫学和肿瘤学,神经科学,

应用

反应种属

Human

目标/特异性

Specificity/Sensitivity

CD8α (D8A8Y) Rabbit mAb recognizes endogenous levels of total CD8α protein. Monkey species reactivity is Immunohistochemistry (Paraffin) only.

Species Reactivity:

Human, Monkey

敏感性

Endogenous

背景

Cluster of Differentiation 8 (CD8) is a disulphide-linked heterodimer consisting of the unrelated α and β subunits. Each subunit is a glycoprotein composed of a single extracellular Ig-like domain, a polypeptide linker, a transmembrane part and a short cytoplasmic tail. On T cells, CD8 is the coreceptor for the T cell receptor (TCR), and these two distinct structures recognize the Antigen–Major Histocompatibility Complex (MHC). Specifically, the Ig-like domain of CD8α interacts with the α3-domain of the MHC class I molecule. CD8 ensures specificity of the TCR–antigen interaction, prolongs the contact between the T cell and the antigen presenting cell, and the α chain recruits the tyrosine kinase Lck, which is essential for T cell activation (1). 1.Zamoyska, R. (1994) Immunity 1, 243-46.

研究领域

癌症,纤维化,免疫学和肿瘤学,神经科学,

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #81575.

数据库链接

Entrez-Gene ID

925

UniProt ID

P01732

参考图片

Flow cytometric analysis of fixed and permeabilized human whole blood using CD8α (D8A8Y) Rabbit mAb co-stained with CD4-PE, showing a distinct CD8α positive population with no cross reactivity to CD4 positive cells. Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4414 was used as a secondary antibody.

Western blot analysis of extracts from MOLT-4 and PC-3 cells using CD8α (D8A8Y) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower).

Immunohistochemical analysis of paraffin-embedded human Crohn's diseased colon using CD8a (D8A8Y) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using CD8a (D8A8Y) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human lymphoma using CD8a (D8A8Y) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded normal rhesus monkey spleen using CD8α (D8A8Y) Rabbit mAb.

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货号：

85336S

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