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咨询Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
9649-CD
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 250 μg/mL in PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Recombinant Feline CD8 alpha Protein, CF Summary
Product Specifications
Ala22-Tyr188, with a C-terminal 6-His tag
Analysis
Background: CD8
CD8 is a dimeric complex made up of two Ig superfamily members (1). The CD8 alpha chain is a type I transmembrane glycoprotein that can dimerize with either itself forming CD8 alpha alpha, or with a 30-35 kDa CD8 beta chain, forming CD8 alpha beta (2). CD8 alpha contains one V-type Ig-like domain in its extracellular region that binds to class I MHC molecules. CD8 alpha beta is a TCR co-receptor, while CD8 alpha alpha promotes T cell survival and differentiation (1, 3). The mature feline CD alpha consists of a 167 amino acid (aa) extracellular domain (ECD), and runs at ~30 kDa on reduced SDS-PAGE. Within the ECD, feline CD8 alpha shares 58%, 47%, 51%, and 69% amino acid sequence identity with human, mouse, porcine, and canine CD8 alpha, respectively.
- Laugel, B. et al. (2011) J. Leukoc. Biol. 90:1089.
- Snow, P.M. and C. Terhorst (1983) J. Biol. Chem. 258:14675.
- Cole, D.K. et al. (2012) Immunology 137:139.