首页抗体一抗内参抗体

BD Pharmingen™ Alexa Fluor® 647 Mouse anti-Hsp60

1/2

BD Pharmingen™ Alexa Fluor® 647 Mouse anti-Hsp60

品牌： BD Pharmingen

下载产品说明书

下载SDS

用小程序，查商品更便捷

对比

咨询

参数

反应种属：

Human (QC Testing), Human,Mouse,Rat (Reactivity Confirmed in Development)

来源宿主：

Mouse IgG1

展开

产品介绍

产品信息

抗原名称

Hsp60

宿主

Mouse IgG1

免疫原

Human Hsp60 Recombinant Protein

简单描述

H eat s hock p roteins (Hsp) are a set of highly conserved proteins that include constitutively expressed (Hsp60, Hsp70, and Hsp90) and stress-induced (Hsp27 and Hsp72) proteins. Hsp60 is localized to the mitochondria, where it promotes mitochondrial protein folding and facilitates proteolytic degradation of misfolded or denatured proteins. It binds Hsp10, which regulates the substrate binding and ATPase activity of Hsp60. In HeLa and Jurkat mitochondria, Hsp60 associates with caspase-3 to form a complex that dissociates and releases from the mitochondria during apoptosis. In addition, Hsp60 accelerates the maturation of procaspase-3 through its ATP-dependent "foldase" activity. In addition to its role in protein folding, Hsp60 has also been implicated in immune function. In macrophages, its binding to the toll-like receptor-4 complex induces production of TNFα and nitric oxide and stimulation of a proinflammatory response. Thus, the protein folding function of Hsp60 is involved in mitochondrial protein folding in both normal and apoptotic cells, while release of Hsp60 during necrosis is thought to stimulate a proinflammatory response.

商品描述

24/HSP60 H eat s hock p roteins (Hsp) are a set of highly conserved proteins that include constitutively expressed (Hsp60, Hsp70, and Hsp90) and stress-induced (Hsp27 and Hsp72) proteins. Hsp60 is localized to the mitochondria, where it promotes mitochondrial protein folding and facilitates proteolytic degradation of misfolded or denatured proteins. It binds Hsp10, which regulates the substrate binding and ATPase activity of Hsp60. In HeLa and Jurkat mitochondria, Hsp60 associates with caspase-3 to form a complex that dissociates and releases from the mitochondria during apoptosis. In addition, Hsp60 accelerates the maturation of procaspase-3 through its ATP-dependent "foldase" activity. In addition to its role in protein folding, Hsp60 has also been implicated in immune function. In macrophages, its binding to the toll-like receptor-4 complex induces production of TNFα and nitric oxide and stimulation of a proinflammatory response. Thus, the protein folding function of Hsp60 is involved in mitochondrial protein folding in both normal and apoptotic cells, while release of Hsp60 during necrosis is thought to stimulate a proinflammatory response.

同种型

Mouse IgG1

克隆号

克隆 24/HSP60 (RUO)

产品详情

Alexa Fluor™ 647

Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor™ 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 670-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

应用

实验应用

Bioimaging (Routinely Tested)

推荐用量

5 µl

反应种属

Human (QC Testing), Human,Mouse,Rat (Reactivity Confirmed in Development)

目标/特异性

Hsp60

制备和贮存

存储溶液

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

保存方式

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

文献

研发参考(4) 1. Ohashi K, Burkart V, Flohe S, Kolb H. Cutting edge: heat shock protein 60 is a putative endogenous ligand of the toll-like receptor-4 complex. J Immunol. 2000; 164(2):558-561. (Biology). 2. Samali A, Cai J, Zhivotovsky B, Jones DP, Orrenius S. Presence of a pre-apoptotic complex of pro-caspase-3, Hsp60 and Hsp10 in the mitochondrial fraction of jurkat cells. EMBO J. 1999; 18(8):2040-2048. (Biology). 3. Venner TJ, Singh B, Gupta RS. Nucleotide sequences and novel structural features of human and Chinese hamster hsp60 (chaperonin) gene families. DNA Cell Biol. 1990; 9(8):545-552. (Biology). 4. Xanthoudakis S, Roy S, Rasper D, et al. Hsp60 accelerates the maturation of pro-caspase-3 by upstream activator proteases during apoptosis. EMBO J. 1999; 18(8):2049-2056. (Biology).

参考图片

Immunofluorescent staining of human cell lines. HeLa cells (ATCC CCL-2) were cultured, fixed, permeabilized with cold methanol, stained with Alexa Fluor® 647 Mouse anti-Hsp60 (pseudo-colored green), and counter-stained with Hoechst 33342 (pseudo-colored blue) according to the Recommended Assay Procedure. The images were captured on a BD Pathway™ 855 Bioimager System with 20x (left panel) and 40X (right panel) objectives and merged using BD Attovision™ software. This antibody also stains A549 (ATCC CCL-185) and U-2 OS (ATCC HTB-96) cells. Triton X-100 is not recommended as a permeabilization agent with this antibody conjugate.

声明：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。

货号：

558684

一键复制

询价

100Tst

选择数量

当前规格1件起购

配送至

预计3-4周送达，快递: 免运费，若需干冰额外收费

询价大包装询价