Chicken Hydrocortisone ELISA Kit

Principle of the Assay: HYD ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HYD antibody and an HYD-HRP conjugate. The assay sample and buffer are incubated together with HYD-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HYD concentration since HYD from samples and HYD-HRP conjugate compete for the anti-HYD antibody binding site. Since the number of sites is limited, as more sites are occupied by HYD from the sample, fewer sites are left to bind HYD-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HYD concentration in each sample is interpolated from this standard curve.

Chicken

Competitive

Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate