ChIP-Grade Protein G Agarose Beads

Chromatin immunoprecipitations were performed using digested chromatin from HeLa cells and either Histone H3 (D2B12) XP® Rabbit mAb (ChIP Formulated) #4620 (lane 2), Rpb1 CTD (4H8) Mouse mAb #2629 (lane 3), Di-Methyl Histone H3 (Lys9) Antibody #9753 (lane 4), or Normal Rabbit IgG #2729 (lane 5). Purified DNA was analyzed by standard PCR methods using SimpleChIP® Human RPL30 Exon 3 Primers #7014, SimpleChIP® Human MyoD1 Exon 1 Primers #4490, and SimpleChIP® Human α Satellite Repeat Primers #4486. PCR products were observed for each primer set in the input sample (lane 1) and various protein-specific immunoprecipitations, but not in the immunoprecipitation using Normal Rabbit IgG #2729 (lane 5).采用消化好的HeLa细胞的染色质和Histone H3 (D2B12) XP ® Rabbit mAb (ChIP Formulated) #4620 (泳道 2), Rpb1 CTD (4H8) Mouse mAb #2629 (泳道 3), Di-Methyl Histone H3 (Lys9) Antibody #9753 (泳道 4), or Normal Rabbit IgG #2729 (lane 5)进行染色质免疫沉淀。用标准PCR对纯化的DNA进行分析，所用引物分别为SimpleChIP™ Human RPL30 Exon 3 Primers #7014、SimpleChIP™ Human MyoD1 Exon 1 Primers #4490、和 SimpleChIP™ Human α Satellite Repeat Primers #4486。针对每个引物的PCR产物可以从所加入的样本和不同的蛋白特异性的免疫沉淀中观察到（泳道1），但在使用Normal Rabbit IgG #2729 的免疫沉淀中则没有观察到PCR产物（泳道5）。

Chromatin immunoprecipitations were performed using digested chromatin from HeLa cells and the indicated antibodies. Purified DNA was analyzed by quantitative real-time PCR, using SimpleChIP® Human RPL30 Exon 3 Primers #7014, SimpleChIP® Human MyoD1 Exon 1 Primers #4490, and SimpleChIP® Human α Satellite Repeat Primers #4486. The relative abundance of each DNA sequence enriched by protein-specific immunoprecipitations is compared to the amount of the same DNA sequence enriched by the non-specific Normal Rabbit IgG #2729 (background).采用消化好的HeLa细胞的染色质和已知抗体进行染色质免疫沉淀。用定量Real-Time PCR对纯化的DNA进行分析，所用引物分别为SimpleChIP™ Human RPL30 Exon 3 Primers #7014、 SimpleChIP™ Human MyoD1 Exon 1 Primers #4490、 和SimpleChIP™ Human α Satellite Repeat Primers #4486。采用蛋白特异性的免疫沉淀所得出的每一条DNA序列的相对含量与非特异性Normal Rabbit IgG #2729 (背景)所测得相同的DNA序列的含量作比较。