CST_Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb_NONE_优宁维(univ)在线
Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb
货号: 9664L 品牌: CST
规格: 300ul
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价格: ¥10920.00

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产品信息
来源纯化
使用与人 caspase-3 Asp175 周围的氨基末端残基相对应的合成肽对动物进行免疫接种来产生单克隆抗体。
内毒素水平
牛 , 犬 , 猪
应用
预测反应种属
牛 , 犬 , 猪
敏感性
内源性
背景
背景
Caspase-3 (CPP-32, Apoptain, Yama, SCA-1) 是一个凋亡的关键执行者,因为它对许多关键的蛋白起着部分或全部的裂解作用,比如核酶多聚 (ADP-ribose) 聚合酶 (PARP) (1)。caspase-3 的激活需要经过其未活化的酶原形式转变为活化的 p17 和 p12 片段的蛋白水解过程。caspase-3 的剪切需要位于 P1 位点的天门冬氨酸残基 (2)。 Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4. Nicholson, D.W. et al. (1995) Nature 376, 37-43.
分子量
17, 19
Entrez-Gene ID
836
UniProt ID
P42574

参考图片

Immunoprecipitation of extracts from Jurkat cells, untreated or etoposide-treated (25uM, 5hrs), using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb. Western blot was performed using the same antibody.

免疫沉淀分析Jurkat 细胞的提取物,未处理或 etoposide (依托泊苷)(25uM, 5hrs)处理,采用Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗,然后用同样抗体进行western blot 分析。

Immunohistochemical staining of paraffin-embedded mouse embryo, showing cytoplasmic localization in apoptotic cells, using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb.

免疫组化分析石蜡包埋小鼠胚胎,显示凋亡细胞中裂解的caspase-3定位胞浆,抗体采用 Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗。

Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with etoposide #2200 (green), using Cleaved Caspase-3(Asp175) (5A1E) Rabbit mAb compared to a nonspecific negative control antibody (red).

Immunohistochemical analysis using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb on SignalSlide (TM) Cleaved Caspase-3 IHC Controls #8104 (paraffin-embedded Jurkat cell, untreated (left) or etoposide-treated (right)).

免疫组化分析,用 SignalSlide (TM) Cleaved Caspase-3 IHC Controls #8104(石蜡包埋的Jurkat 细胞,未处理(左)或 etoposide 处理(右)),采用 Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗。

Western blot analysis of extracts from C6 (rat), NIH/3T3 (mouse), and Jurkat (human) cells, untreated or treated with staurosporine (1uM, 3hrs) or etoposide (25uM, 5hrs) as indicated, using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb.

Western blot 分析未处理或 staurosporine (星胞菌素) (1uM, 3hrs) 或 etoposide (依托泊苷)(25uM, 5hrs)处理的C6 (大鼠源)、NIH/3T3 (小鼠源)和Jurkat (人源) 细胞。抗体采用Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗。

Immunohistochemical analysis of paraffin-embedded mouse embryo, using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb in the presence of control peptide (left) or Cleaved Caspase-3 (Asp175) Blocking Peptide (#1050) (right).

免疫组化分析石蜡包埋的小鼠胚胎,抗体采用Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗,并用对照肽段(左图)或 Cleaved Caspase-3 (Asp175) 封闭肽段 (#1050) 对照(右图)。

Confocal immunofluorescent images of HT-29 cells, untreated (left) or Staurosporine treated (right) labeled with Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb#9953 (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

共聚焦免疫荧光分析未处理(左)或 Staurosporine (星胞菌素)处理(右)的HT-29 细胞,抗体采用 Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗#9953(绿色)。微丝用Alexa Fluor® 555 phalloidin(红色)标记。蓝色伪彩=DRAQ5® #4084 (DNA 荧光染料)。

Immunohistochemical analysis of frozen H1650 xenograft, using Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb.

免疫组化分析 H1650 xenograft 冰冻切片,抗体采用Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb 兔单抗。

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