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Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb
品牌： CST
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对比
咨询分子量：
17, 19
反应种属：
Human,Mouse,Rat,Monkey,
产品介绍
产品介绍
产品信息
荧光素标记
Unconjugated
抗原名称
Cleaved Caspase-3
来源纯化
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to amino-terminal residues adjacent to Asp175 of human caspase-3.
宿主
Rabbit
商品描述
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:2000 |
| Immunofluorescence (Immunocytochemistry) | 1:400 - 1:1600 |
| Flow Cytometry (Fixed/Permeabilized) | 1:6400 |
同种型
Rabbit IgG
分子量
17, 19
内毒素水平
牛 , 犬 , 猪
研究领域
癌症,细胞生物学,纤维化,神经科学,
应用
反应种属
Human,Mouse,Rat,Monkey,
预测反应种属
Bovine,Dog,Pig,
目标/特异性
Specificity/Sensitivity
Cleaved Caspase-3 (Asp175) (5A1E) Rabbit mAb detects endogenous levels of the large fragment (17/19 kDa) of activated caspase-3 resulting from cleavage adjacent to Asp175. This antibody does not recognize full-length caspase-3 or other cleaved caspases. Non-specific labeling may be observed by immunofluorescence in specific sub-types of healthy cells in fixed-frozen tissues (e.g. pancreatic alpha-cells). Cytoplasmic background may be observed in human and monkey samples.
Species Reactivity:
Human, Mouse, Rat, Monkey
敏感性
Endogenous
背景
背景
Caspase-3 (CPP-32, Apopain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2).
1.Fernandes-Alnemri, T. et al. (1994) J Biol Chem 269, 30761-4.
2.Nicholson, D.W. et al. (1995) Nature 376, 37-43.
研究领域
癌症,细胞生物学,纤维化,神经科学,
翻译后修饰
cleaved
制备和贮存
保存方式
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #94530.
数据库链接
Entrez-Gene ID
836
UniProt ID
P42574
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