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产品介绍
产品介绍
产品信息
荧光素标记
抗原名称
Cleaved Caspase-9
来源纯化
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp315 of human caspase-9. Antibodies are purified by protein A and peptide affinity chromatography.
宿主
Rabbit
商品描述
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
分子量
35
研究领域
癌症,细胞生物学,纤维化,神经科学,
应用
反应种属
Human
目标/特异性
Specificity/Sensitivity
Cleaved Caspase-9 (Asp315) Antibody detects endogenous levels of the 35 kDa large fragment of caspase-9 following cleavage at aspartic acid 315. The antibody does not react with full length caspase-9 or any other cleaved caspases.
Species Reactivity:
Human
敏感性
Endogenous
背景
背景
Caspase-9 (ICE-LAP6, Mch6) is an important member of the cysteine aspartic acid protease (caspase) family (1,2). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with the 47 kDa procaspase-9/Apaf-1. Apaf-1 mediated activation of caspase-9 involves intrinsic proteolytic processing, resulting in cleavage at Asp315 and producing a p35 subunit. Another cleavage occurs at Asp330, producing a p37 subunit that can serve to amplify the apoptotic response (3-6). Cleaved caspase-9 further processes other caspase members, including caspase-3 and caspase-7, to initiate a caspase cascade, which leads to apoptosis (7-10).
1.Duan, H. et al. (1996) J. Biol. Chem. 271, 16720-16724.
2.Srinivasula, S. M. et al. (1996) J. Biol. Chem. 271, 27099-27106.
3.Liu, X. et al. (1996) Cell 86, 147-157.
4.Li, P. et al. (1997) Cell 91, 479-489.
5.Zou, H. et al. (1999) J. Biol. Chem. 274, 11549-11556.
6.Srinivasula, S.M. et al. (1998) Mol Cell 1, 949-57.
7.Deveraux, Q. L. et al. (1998) EMBO J. 17, 2215-2223.
8.Slee, E. A. et al. (1999) J. Cell Biol. 144, 281-292.
9.Sun, X.M. et al. (1999) J Biol Chem 274, 5053-60.
10.MacFarlane, M. et al. (1997) J. Cell Biol. 137, 469-479.
研究领域
癌症,细胞生物学,纤维化,神经科学,
翻译后修饰
cleaved
制备和贮存
保存方式
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
数据库链接
Entrez-Gene ID
842
UniProt ID
P55211
参考图片
Western blot analysis of extracts from Jurkat cells, untreated or cytochrome c-treated (0.25 mg/ml), and HeLa cells, untreated, staurosporine-treated (1 µM), or cytochrome c-treated (0.25 mg/ml), using Cleaved Caspase-9 (Asp315) Antibody (upper) or Caspase-9 Antibody #9502 (lower).
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