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Cleaved Gasdermin D (Asp276) (E3E3P) Rabbit mAb

Cleaved Gasdermin D (Asp276) (E3E3P) Rabbit mAb

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分子量:
31
反应种属:
Mouse
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产品介绍
产品介绍
产品信息
荧光素标记
抗原名称
Cleaved Gasdermin D
来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp276 of mouse Gasdermin D protein.

宿主
Rabbit
商品描述

Product Usage Information

ApplicationDilution
Western Blotting1:1000
同种型
Rabbit IgG
分子量
31
研究领域
癌症,细胞生物学,纤维化,神经科学,
应用
反应种属
Mouse
目标/特异性

Specificity/Sensitivity

Cleaved Gasdermin D (Asp276) (E3E3P) Rabbit mAb recognizes endogenous levels of the amino fragment of mouse Gasdermin D protein only when cleaved at Asp276.

Species Reactivity:

Mouse

敏感性
Endogenous
背景
背景
Gasdermin D (GSDMD), a member of the gasdermin family that includes GSDMA, GSDMB, and GSDMC, has been reported to have a critical role as a downstream effector of pyroptosis (1,2). Pyroptosis is a lytic type of cell death triggered by inflammasomes, multiprotein complexes assembled in response to pathogen-associated molecular patterns (PAMPs) or danger-associated molecular patterns (DAMPs) that result in the activation of caspase-1 and subsequent cleavage of pro-inflammatory cytokines IL-1β and IL-18 (3). Gasdermin D was identified by two independent groups as a substrate of inflammatory caspases, caspase-1 and caspase-11/4/5, producing two fragments: GSDMD-N and GSDMD-C. Cleavage results in release of an intramolecular inhibitory interaction between the N- and C-terminal domains, allowing the N-terminal fragment GSDMD-N to initiate pyroptosis through the formation of pores on the plasma membrane (4-7). 1.Kayagaki, N. et al. (2015) Nature 526, 666-71. 2.Shi, J. et al. (2015) Nature 526, 660-5. 3.Broz, P. and Dixit, V.M. (2016) Nat Rev Immunol 16, 407-20. 4.Aglietti, R.A. et al. (2016) Proc Natl Acad Sci U S A 113, 7858-63. 5.Ding, J. et al. (2016) Nature 535, 111-6. 6.Liu, X. et al. (2016) Nature 535, 153-8. 7.Sborgi, L. et al. (2016) EMBO J 35, 1766-78.
研究领域
癌症,细胞生物学,纤维化,神经科学,
翻译后修饰
cleaved
制备和贮存
保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

数据库链接
Entrez-Gene ID
69146
UniProt ID
Q9D8T2

参考图片

Western blot analysis of extracts from mouse bone marrow derived macrophages (mBMDM), untreated (-) or treated with Lipopolysaccharides (LPS) #14011 (50 ng/ml, 4 hr; +) followed by Nigericin (sodium salt) #66419 (15 μM, indicated times; +), using Cleaved Gasdermin D (Asp276) (E3E3P) Rabbit mAb (upper) or GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

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货号:
10137S
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询价
供应商现货
20μl
100ul
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