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Cleaved-IL-1beta (Asp116) (D3A3Z) Rabbit mAb

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Cleaved-IL-1beta (Asp116) (D3A3Z) Rabbit mAb

品牌： CST

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参数

分子量：

反应种属：

Human

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产品介绍

产品信息

抗原名称

Cleaved-IL-1beta

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp116 of human IL-1β protein.

宿主

Rabbit

简单描述

Monoclonal Antibody for studying IL1 beta (Asp116) cleaved. Cited in 80 publications. Validated for Western Blotting, Immunofluorescence (Immunocytochemistry). Highly specific and rigorously validated in-house, Cleaved-IL-1β (Asp116) (D3A3Z) Rabbit Monoclonal Antibody (CST #83186) is ready to ship.

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunofluorescence (Immunocytochemistry)	1:800 - 1:3200

分子量

研究领域

纤维化,免疫学和肿瘤学,神经科学

应用

反应种属

Human

目标/特异性

Specificity/Sensitivity

Cleaved-IL-1β (Asp116) (D3A3Z) Rabbit mAb recognizes endogenous levels of mature IL-1β protein only when cleaved at Asp116.

Species Reactivity:

Human

敏感性

Endogenous

背景

Interleukin-1β (IL-1β), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses (1). It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets (2-6). Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form (7,8). Detection of the 17 kDa mature form of IL-1β is a good indicator of caspase-1 activity. 1.Dinarello, C.A. (1998) Int Rev Immunol 16, 457-99. 2.Burns, K. et al. (1998) J Biol Chem 273, 12203-9. 3.Cao, Z. et al. (1996) Nature 383, 443-6. 4.Cao, Z. et al. (1996) Science 271, 1128-31. 5.Wesche, H. et al. (1997) Immunity 7, 837-47. 6.Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-6. 7.Thornberry, N.A. et al. (1992) Nature 356, 768-74. 8.Cerretti, D.P. et al. (1992) Science 256, 97-100.

研究领域

纤维化,免疫学和肿瘤学,神经科学

翻译后修饰

Cleaved

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

数据库链接

Entrez-Gene ID

3553

UniProt ID

P01584

参考图片

Confocal immunofluorescent analysis of THP-1 cells, differentiated with TPA #4174 (80 nM, 24 hr) and subsequently treated with (right) or without (left) Lipopolysaccharides (LPS) #14011 (1 μg/ml, 6 hr), using Cleaved-IL-1β (Asp116) (D3A3Z) Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Blue pseudocolor = DRAQ5^® #4084 (fluorescent DNA dye).

Western blot analysis of recombinant Human Interleukin-1β (hIL-1β) #8900 using Cleaved-IL-1β (Asp116) (D3A3Z) Rabbit mAb.

Western blot analysis of extracts from cells or media collected from THP-1 cells, differentiated with TPA #4147 (80 nM, overnight) and subsequently treated with (+) or without (-) Lipopolysaccharides (LPS) #14011 (1 μg/ml, 6 hr), using Cleaved-IL-1β (Asp116) (D3A3Z) Rabbit mAb.

Immunoprecipitation of Cleaved-IL-1β (Asp116) from extracts of THP-1 cells differentiated with TPA #4147 (80 nM, overnight) followed by treatment with Lipopolysaccharides (LPS) #14011 (1 μg/ml, 6 hr). Lane 1 is 10% input, lane 2 is precipitated with Rabbit (DA1E) mAb IgG XP^® Isotype Control #3900, and lane 3 is precipitated with Cleaved-IL-1β (Asp116) (D3A3Z) Rabbit mAb. Western blot was performed using Cleaved-IL-1β (Asp116) (D3A3Z) Rabbit mAb.

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货号：

83186S

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