Cleaved-IL-1-beta (Asp116) (D3A3Z) Rabbit mAb

Interleukin-1β (IL-1β), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses (1). It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets (2-6). Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form (7,8). Detection of the 17 kDa mature form of IL-1β is a good indicator of caspase-1 activity. 1.Dinarello, C.A. (1998) Int Rev Immunol 16, 457-99. 2.Burns, K. et al. (1998) J Biol Chem 273, 12203-9. 3.Cao, Z. et al. (1996) Nature 383, 443-6. 4.Cao, Z. et al. (1996) Science 271, 1128-31. 5.Wesche, H. et al. (1997) Immunity 7, 837-47. 6.Ninomiya-Tsuji, J. et al. (1999) Nature 398, 252-6. 7.Thornberry, N.A. et al. (1992) Nature 356, 768-74. 8.Cerretti, D.P. et al. (1992) Science 256, 97-100.