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Recombinant Human CLEC10A/CD301 Protein, CF (50 ug)
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咨询Recombinant Human CLEC10A/CD301 Protein, CF Summary
- R&D Systems NS0-derived Recombinant Human CLEC10A/CD301 Protein (4888-CL)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Product Specifications
Gln61-His316, with an N-terminal 6-His tag
Analysis
Product Datasheets
4888-CL
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
4888-CL
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 200 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Reconstitution Calculator
Background: CLEC10A/CD301
CLEC10A, also known as macrophage galactose/N-acetyl-galactosamine (GalNAc) specific lectin (MGL), CD301, DC-ASGPR, and HML, is a 40 kDa type II transmembrane glycoprotein that belongs to the C-type lectin family (1). Human and rat carry a single gene for CLEC10A/MGL, while mouse has two closely related MGL1 and MGL2 genes. Human CLEC10A/MGL consists of a 39 amino acid (aa) cytoplasmic region, a 21 aa transmembrane segment and a 256 aa extracellular domain (ECD) with one carbohydrate recognition domain (CRD) and a neck region (2). Within the CRD, human CLEC10A/MGL shares 64% - 70% aa sequence identity with mouse MGL1, mouse MGL2, and rat MGL. Alternate splicing generates multiple isoforms of human CLEC10A/MGL with 27 aa, 3 aa, and/or 4 aa deletions within the ECD (3, 4). CLEC10A/MGL is expressed on immature myleloid dendritic cells and alternatively activated (tolerogenic) macrophages and is upregulated by the immunosuppressant dexamethasone (3 - 7). CLEC10A/MGL selectively binds and internalizes terminal nonsialylated alpha - or beta -linked GalNAc moieties on O-linked carbohydrates, including the Tn carcinoma antigen (2 - 4, 8, 9). Similar ligand preference is exhibited by mouse MGL2 but not MGL1 (10). CLEC10A/MGL expressed on tolerogenic dendritic cells binds carbohydrate determinants on CD45 (RA, RB, and RC but not RO isoforms) expressed by T, NK, and B cells (6). This interaction inhibits effector T cell activation and induces their apoptosis (6). CLEC10A/MGL also binds the GP envelope glycoprotein on Marburg and Ebola viruses and enhances viral entry and infectivity (11).
- Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
- Suzuki, N. et al. (1996) J. Immunol. 156:128.
- Higashi, N. et al. (2002) J. Biol. Chem. 277:20686.
- Valladeau, J. et al. (2001) J. Immunol. 167:5767.
- van Vliet, S.J. et al. (2006) Immunobiology 211:577.
- van Vliet, S.J. et al. (2006) Nat. Immunol. 7:1200.
- Raes, G. et al. (2005) J. Leukoc. Biol. 77:321.
- van Vliet, S.J. et al. (2005) Int. Immunol. 17:661.
- Higashi, N. et al. (2002) Int. Immunol. 14:545.
- Tsuiji, M. et al. (2002) J. Biol. Chem. 277:28892.
- Takada, A. et al. (2004) J. Virol. 78:2943.
Background: CLEC10A/CD301
CLEC10A, also known as macrophage galactose/N-acetyl-galactosamine (GalNAc) specific lectin (MGL), CD301, DC-ASGPR, and HML, is a 40 kDa type II transmembrane glycoprotein that belongs to the C-type lectin family (1). Human and rat carry a single gene for CLEC10A/MGL, while mouse has two closely related MGL1 and MGL2 genes. Human CLEC10A/MGL consists of a 39 amino acid (aa) cytoplasmic region, a 21 aa transmembrane segment and a 256 aa extracellular domain (ECD) with one carbohydrate recognition domain (CRD) and a neck region (2). Within the CRD, human CLEC10A/MGL shares 64% - 70% aa sequence identity with mouse MGL1, mouse MGL2, and rat MGL. Alternate splicing generates multiple isoforms of human CLEC10A/MGL with 27 aa, 3 aa, and/or 4 aa deletions within the ECD (3, 4). CLEC10A/MGL is expressed on immature myleloid dendritic cells and alternatively activated (tolerogenic) macrophages and is upregulated by the immunosuppressant dexamethasone (3 - 7). CLEC10A/MGL selectively binds and internalizes terminal nonsialylated alpha - or beta -linked GalNAc moieties on O-linked carbohydrates, including the Tn carcinoma antigen (2 - 4, 8, 9). Similar ligand preference is exhibited by mouse MGL2 but not MGL1 (10). CLEC10A/MGL expressed on tolerogenic dendritic cells binds carbohydrate determinants on CD45 (RA, RB, and RC but not RO isoforms) expressed by T, NK, and B cells (6). This interaction inhibits effector T cell activation and induces their apoptosis (6). CLEC10A/MGL also binds the GP envelope glycoprotein on Marburg and Ebola viruses and enhances viral entry and infectivity (11).
- Zelensky, A.N. and J.E. Gready (2005) FEBS J. 272:6179.
- Suzuki, N. et al. (1996) J. Immunol. 156:128.
- Higashi, N. et al. (2002) J. Biol. Chem. 277:20686.
- Valladeau, J. et al. (2001) J. Immunol. 167:5767.
- van Vliet, S.J. et al. (2006) Immunobiology 211:577.
- van Vliet, S.J. et al. (2006) Nat. Immunol. 7:1200.
- Raes, G. et al. (2005) J. Leukoc. Biol. 77:321.
- van Vliet, S.J. et al. (2005) Int. Immunol. 17:661.
- Higashi, N. et al. (2002) Int. Immunol. 14:545.
- Tsuiji, M. et al. (2002) J. Biol. Chem. 277:28892.
- Takada, A. et al. (2004) J. Virol. 78:2943.