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COL1A1 (E8F4L) XP ® Rabbit mAb

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COL1A1 (E8F4L) XP ® Rabbit mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

抗原名称

COL1A1

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe1196 of human COL1A1 protein.

宿主

Rabbit

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
IHC Leica Bond	1:100 - 1:400
Immunohistochemistry (Paraffin)	1:50 - 1:200
Immunofluorescence (Frozen)	1:100 - 1:400
Immunofluorescence (Immunocytochemistry)	1:100 - 1:400

同种型

Rabbit IgG

分子量

220

研究领域

癌症,细胞生物学,纤维化,神经科学,

应用

反应种属

Human,Mouse,

目标/特异性

Specificity/Sensitivity

COL1A1 (E8F4L) XP Rabbit mAb recognizes endogenous levels of total COL1A1 protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

敏感性

Endogenous

背景

Type 1 collagen is the most abundant collagen in many human tissues, including bone, skin, and tendons. It is a trimeric complex composed of two molecules of COL1A1 (alpha-1 type 1 collagen) and one molecule of COL1A2 (alpha-2 type 1 collagen) (1-3). The expression levels of COL1A1 are regulated by multiple mechanisms, including mRNA stability, translation, and post-translational modification (3-5). Overexpression of COL1A1 has been positively associated with tissue fibrosis disorders, including systemic sclerosis (6), while loss-of-function mutations in the COL1A1 gene are a major causative factor for osteogenesis imperfecta (brittle bone disease) (7). Notably, COL1A1 expression levels have also been associated with tumor development in gastric, lung, thyroid, and breast cancers. Research studies suggest that upregulation of COL1A1 can generate a modified extracellular matrix environment that promotes cancer cell survival, proliferation, metastasis, and invasion (8-11). 1.Prockop, D.J. and Kivirikko, K.I. (1995) Annu Rev Biochem 64, 403-34. 2.Chang, S.W. et al. (2012) Biophys J 102, 640-8. 3.Zhang, Y. and Stefanovic, B. (2016) Int J Mol Sci 17, 419. 4.Parsons, C.J. et al. (2011) J Biol Chem 286, 8609-19. 5.Cai, L. et al. (2010) J Mol Biol 395, 309-26. 6.Jimenez, S.A. and Saitta, B. (1999) Springer Semin Immunopathol 21, 397-414. 7.Forlino, A. et al. (2011) Nat Rev Endocrinol 7, 540-57. 8.Li, J. et al. (2016) World J Surg Oncol 14, 297. 9.Oleksiewicz, U. et al. (2017) J Cancer Res Clin Oncol 143, 1133-41. 10.Barcus, C.E. et al. (2017) Breast Cancer Res 19, 9. 11.Jolly, L.A. et al. (2016) Cancer Res 76, 1804-13.

研究领域

癌症,细胞生物学,纤维化,神经科学,

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #81375.

数据库链接

Entrez-Gene ID

1277

UniProt ID

P02452

参考图片

Confocal immunofluorescent analysis of U-118 MG cells (left, positive) and HT-29 cells (right, negative) using COL1A1 (E8F4L) XP® Rabbit mAb (green). Actin filaments were labeled with DyLight™ 554 Phalloidin #13054 (red). Samples were mounted in ProLong® Gold Antifade Reagent with DAPI #8961 (blue).

Western blot analysis of extracts from various cell lines using COL1A1 (E8F4L) XP® Rabbit mAb (upper) and GAPDH (D16H11) XP® Rabbit mAb #5174 (lower).

Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using COL1A1 (E8F4L) XP® Rabbit mAb performed on the Leica® BOND™ Rx.

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using COL1A1 (E8F4L) XP® Rabbit mAb performed on the Leica® BOND™ Rx.

Immunohistochemical analysis of paraffin-embedded human prostate carcinoma using COL1A1 (E8F4L) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded normal human kidney using COL1A1 (E8F4L) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma (left), renal cell carcinoma (middle) or prostate carcinoma (right) using COL1A1 (E8F4L) XP® Rabbit mAb (top) or COL1A1 (E3E1X) Mouse mAb #66948 (bottom). These two antibodies detect independent, unique epitopes on human COL1A1. The similar staining patterns obtained with both antibodies help to confirm the specificity of the staining.

Immunohistochemical analysis of paraffin-embedded A20 syngeneic tumor using COL1A1 (E8F4L) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded GL261 syngeneic tumor using COL1A1 (E8F4L) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded mouse liver using COL1A1 (E8F4L) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded mouse lung using COL1A1 (E8F4L) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded rhesus monkey heart using COL1A1 (E8F4L) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded rat colon using COL1A1 (E8F4L) XP® Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human esophageal adenocarcinoma using COL1A1 (E8F4L) XP® Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).

Immunohistochemical analysis of paraffin-embedded U-118 MG cell pellet (left, positive) or HT-29 cell pellet (right, negative) using COL1A1 (E8F4L) XP® Rabbit mAb.

Confocal immunofluorescent analysis of fixed frozen mouse spleen labeled with COL1A1 (E8F4L) XP® Rabbit mAb (green), α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #76113 (red), and ProLong Gold Antifade Reagent with DAPI #8961 (blue).

Confocal immunofluorescent analysis of fixed frozen mouse colon labeled with COL1A1 (E8F4L) XP® Rabbit mAb (green), β-Catenin (D10A8) XP® Rabbit mAb (Alexa Fluor® 555 Conjugate) #83539 (yellow), α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #76113 (red), and ProLong Gold Antifade Reagent with DAPI #8961 (blue).

Confocal immunofluorescent analysis of fixed frozen mouse liver labeled with COL1A1 (E8F4L) XP® Rabbit mAb (green), α-Smooth Muscle Actin (D4K9N) XP® Rabbit mAb (Alexa Fluor® 647 Conjugate) #76113 (red) and ProLong Gold Antifade Reagent with DAPI #8961 (blue).

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货号：

72026T

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