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COL5A1 (E6U9W) Rabbit mAb

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COL5A1 (E6U9W) Rabbit mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro364 of human COL5A1 protein.

宿主

Rabbit

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:100
IHC Leica Bond	1:50 - 1:200
Immunohistochemistry (Paraffin)	1:50 - 1:200
Immunofluorescence (Immunocytochemistry)	1:200 - 1:800

同种型

Rabbit IgG

分子量

250

应用

目标/特异性

Specificity/Sensitivity

COL5A1 (E6U9W) Rabbit mAb recognizes endogenous levels of total COL5A1 protein. Nonspecific staining was observed in skeletal muscle by immunohistochemistry.

Species Reactivity:

Human

敏感性

Endogenous

背景

The Extracellular Matrix (ECM) is a complex network of macromolecules that provides structural tissue support to cells in the basement membrane and interstitial matrix. It is composed of many molecules including proteins, glycoproteins, proteoglycans, and polysaccharides (1,2). One of the major proteins that comprises the ECM, and the human body, is collagen. Collagens are a large family of proteins. They are trimeric molecules comprised of three alpha polypeptide chains that form a triple helix structure that is characteristic of all collagens (3). The large family of collagens is divided into three subgroups: the fibrillar collagens, the non-fibril forming collagens, and the fibril-associated collagens. These sub groups differ in their structure and supramolecular assembly (3). COL5A1 is a minor fibrillar collagen expressed broadly across tissues. It participates in bone and cartilage development, and defects in the COL5A1 gene leads to Ehlers-Danlos Syndrome, a condition that affects the integrity of bones, cartilage, and skin (4,5). Several proteomics, gene profiling, and biochemical studies have indicated that aberrant COL5A1 may play a malignant role in various types of cancer including breast, renal, colon, prostate, lung, and others (6-10). 1.Barkan, D. et al. (2010) Eur J Cancer 46, 1181-8. 2.Hynes, R.O. (2009) Science 326, 1216-9. 3.Ricard-Blum, S. (2011) Cold Spring Harb Perspect Biol 3, a004978. 4.Wu, Y.F. et al. (2010) Matrix Biol 29, 701-6. 5.Kuroda, Y. et al. (2018) Congenit Anom (Kyoto) 58, 191-193. 6.Wang, S. et al. (2019) Theranostics 9, 4342-4353. 7.Feng, G. et al. (2019) Cancer Manag Res 11, 1263-1274. 8.Wu, M. et al. (2019) Oncol Rep 42, 151-175. 9.Chen, H.C. et al. (2019) J Oral Pathol Med 48, 468-476. 10.Nguyen, E.V. et al. (2019) Mol Cell Proteomics 18, 1410-1427.

研究领域

癌症,细胞生物学,纤维化,神经科学,

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #80085.

数据库链接

Entrez-Gene ID

1289

UniProt ID

P20908

参考图片

Confocal immunofluorescent analysis of SF539 cells (left, positive) or ACHN cells (right, negative) using COL5A1 (E6U9W) Rabbit mAb (green), S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 647 Conjugate) #5548 (red), and DAPI #4083 (blue).

Western blot analysis of extracts from Saos-2 and SK-N-SH cells using COL5A1 (E6U9W) Rabbit mAb (upper) and β-Actin (D6A8) Rabbit mAb #8457 (lower). The differential in COL5A1 expression between Saos-2 and SK-N-SH cells is consistent with their reported molecular expression profiles (www.biogps.org), confirming specificity of the antibody for COL5A1.

Immunoprecipitation of COL5A1 protein from SF-539 cell extracts. Lane 1 is 10% input, lane 2 is Rabbit (DA1E) mAb IgG XP® Isotype Control #3900, and lane 3 is COL5A1 (E6U9W) Rabbit mAb. Western blot analysis was performed using COL5A1 (E6U9W) Rabbit mAb. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as the secondary antibody.

Immunohistochemical analysis of paraffin-embedded human esophageal carcinoma using COL5A1 (E6U9W) Rabbit mAb performed on the Leica® BOND™ Rx.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using COL5A1 (E6U9W) Rabbit mAb performed on the Leica® BOND™ Rx.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using COL5A1 (E6U9W) Rabbit mAb performed on the Leica® BOND™ Rx.

Immunohistochemical analysis of paraffin-embedded human ovarian adenocarcinoma using COL5A1 (E6U9W) Rabbit mAb performed on the Leica® BOND™ Rx.

Immunohistochemical analysis of paraffin-embedded human endometrioid adenocarcinoma using COL5A1 (E6U9W) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded normal human placenta using COL5A1 (E6U9W) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded normal human colon using COL5A1 (E6U9W) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human neuroendocrine lung carcinoma using COL5A1 (E6U9W) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).

Immunohistochemical analysis of paraffin-embedded SF-539 cell pellet (left, positive) or ACHN cell pellet (right, negative) using COL5A1 (E6U9W) Rabbit mAb.

Confocal immunofluorescent analysis of U-118 MG cells (left, positive), RD cells (center, positive) or OVCAR-5 cells (right, negative) using COL5A1 (E6U9W) Rabbit mAb (green), S6 Ribosomal Protein (54D2) Mouse mAb (Alexa Fluor® 647 Conjugate) #5548 (red), and DAPI #4083 (blue).

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货号：

86903T

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