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CONNEXIN-43 ANTIBODY
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Reconstitution: 25 µL, 50 µL or 0.2 mL double distilled water (DDW), depending on the sample size. The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20C. The reconstituted solution can be stored at 4C for up to 1 week. For longer periods, small aliquots should be stored at -20C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
靶标信息
Connexin 43 (Cx43) is a member of the gap junction protein family. Connexins assemble as a hexamer and are transported to the plasma membrane to create a hemichannel that can associate with hemichannels on nearby cells to create cell-to-cell channels. Clusters of these channels assemble to make gap junctions. Gap junction communication is important in development and regulation of cell growth. Phosphorylation of Cx43 is important in regulating assembly and function of gap junctions. Ser368 of Cx43 is phosphorylated by protein kinase C (PKC) after activation by phorbol esters, which decreases cell-to-cell communication. Src can interact with and phosphorylate Cx43 to alter gap junction communication. GFAP are membrane-spanning proteins that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Connexin 43 is the major protein of gap junctions in the heart, and gap junctions are thought to have a crucial role in the synchronized contraction of the heart and in embryonic development. Connexin 43 is also targeted by several protein kinases that regulate myocardial cell-cell coupling. A related intron-less connexin 43 pseudogene, GJA1P, has been mapped to chromosome 5. Mutations in the GFAP gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy, oculodentodigital dysplasia and heart malformations. Alternatively spliced transcript variants of GFAP have been found.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: alpha 1 connexin; connexin 43; Connexin-43; Cx-43; Cx43; CXA1; DFNB38; Gap junction 43 kDa heart protein; Gap junction alpha-1 protein; gap junction membrane channel protein alpha 1; gap junction protein, alpha 1, 43kDa; ODD; ODOD; SDTY3; Syndactyly type III
基因别名: AU042049; AVSD3; AW546267; CMDR; Cnx43; connexin43; CX43; Cx43alpha1; Cxn-43; EKVP; Gja-1; GJA1; GJAL; HLHS1; HSS; Npm1; ODDD; PPKCA
UniProt ID:(Human) P17302, (Mouse) P23242, (Rat) P08050
Entrez Gene ID:(Human) 2697, (Mouse) 14609, (Rat) 24392
参考图片
Multiplex staining of Connexin-43 and GFAP in rat cerebellum. Immunohistochemical staining of perfusion-fixed frozen rat brain sections with Guinea pig Anti-Connexin-43 Antibody (#ACC-201-GP), (1:200), followed by goat Anti-guinea pig-AlexaFluor-594 and Anti-GFAP Antibody (#AFP-001), (1:1200), followed by goat Anti-rabbit-AlexaFluor-488. A. Connexin-43 immunoreactivity (red) appears as positive puncta (arrows). B. GFAP immunoreactivity (green) appears along the Bergmann glial processes (arrows point at examples). C. Merge of the two images shows CNX-43 puncta distributed along the Bergmann glial processes. Cell nuclei are stained with DAPI (blue).
Expression of Connexin-43 in mouse brain - Immunohistochemical staining of frozen mouse cerebellum free floating sections using Guinea pig Anti-Connexin-43 Antibody (#ACC-201-GP). CX43 immunoreactivity (red) appears in the Purkinje layer (vertical arrows) and in puncta along dendritic processes (horizontal arrows). Nuclei are stained using DAPI as the counterstain (blue).
Western blot analysis of human T-cell leukemia (Jurkat) (lanes 9 and 11) and human neuroblastoma (SH-SY5Y) (lanes 10 and 12) cell line lysates: - 9,10. Guinea pig Anti-Connexin-43 Antibody (#ACC-201-GP), (1:400). 11,12. Guinea pig Anti-Connexin-43 Antibody , preincubated with Connexin-43 Blocking Peptide (#BLP-CC201).
Western blot analysis of rat brain (lanes 1 and 3), (1:1000) and mouse brain (lanes 2 and 4), (1:200) membranes: - 1,2. Guinea pig Anti-Connexin-43 Antibody (#ACC-201-GP).3,4. Guinea pig Anti-Connexin-43 Antibody , preincubated with Connexin-43 Blocking Peptide (#BLP-CC201).
Western blot analysis of rat (lanes 5 and 7) and mouse (lanes 6 and 8)heart membranes: - 5,6. Guinea pig Anti-Connexin-43 Antibody (#ACC-201-GP), (1:400). 7,8. Guinea pig Anti-Connexin-43 Antibody , preincubated with Connexin-43 Blocking Peptide (#BLP-CC201).