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产品介绍
产品介绍
产品信息
Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean (ng/mL) | 1.3 | 4.5 | 6.51 | 1.11 | 3.42 | 5.54 |
| Standard Deviation | 0.12 | 0.241 | 0.411 | 0.235 | 0.319 | 0.576 |
| CV% | 9.2 | 5.4 | 6.3 | 21.2 | 9.3 | 10.4 |
Recovery
The recovery of Cortisol spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 103 | 87-113 |
| EDTA Plasma (n=4) | 97 | 94-103 |
| Heparin Plasma (n=4) | 98 | 92-107 |
| Saliva (n=4) | 101 | 88-118 |
| Serum (n=4) | 95 | 89-100 |
| Urine (n=4) | 92 | 78-113 |
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Cortisol were serially diluted with calibrator diluent to produce samples with values within the dynamic range of the assay.
Scientific Data
Cortisol Parameter Assay Kit Summary
Assay Length
2.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (20 uL), Urine (10 uL)
Sensitivity
0.111 ng/mL
Assay Range
0.2 - 10 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine)
Specificity
Measures Cortisol levels in various samples
Cross-reactivity
Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.
背景
别名
Compound F,Cortisol,Hydrocortisone
背景
Background: Cortisol
Cortisol, also known as hydrocortisone or compound F, is the primary glucocorticoid produced and secreted by the adrenal cortex. Found either free or bound to corticosteroid-binding globulin (CBG) in the blood, cortisol is involved in glucose homeostasis, inflammation, hypersensitivity, immunosuppression, and disease resistance. Plasma cortisol levels increase in response to stress and abnormal levels are implicated in prostate cancer, depression, and schizophrenia. Cushing's disease is caused by systemic cortisol excess.
Alternate Names:
Compound F; Cortisol; Hydrocortisone
研究领域
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