老带新
COX IV (4D11-B3-E8) Mouse mAb
1/4

COX IV (4D11-B3-E8) Mouse mAb

分享
品牌: CST
pdf 下载产品说明书
pdf 下载SDS
用小程序,查商品更便捷
收藏
对比对比
咨询咨询
分子量:
17
反应种属:
Human,Mouse,Rat,Monkey,
展开
产品介绍
产品介绍
产品信息
荧光素标记
抗原名称
COX IV
来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human COX IV protein.

宿主
Mouse
商品描述

Product Usage Information

ApplicationDilution
Western Blotting1:1000
Immunoprecipitation1:100
Immunohistochemistry (Paraffin)1:50
Immunofluorescence (Immunocytochemistry)1:200
同种型
Mouse IgG1
分子量
17
研究领域
癌症,细胞生物学,纤维化,代谢,神经科学
应用
反应种属
Human,Mouse,Rat,Monkey,
目标/特异性

Specificity/Sensitivity

COX IV (4D11-B3-E8) Mouse mAb recognizes endogenous levels of total COX IV protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

敏感性
Endogenous
背景
背景
Cytochrome c oxidase (COX) is a hetero-oligomeric enzyme consisting of 13 subunits localized to the inner mitochondrial membrane (1-3). It is the terminal enzyme complex in the respiratory chain, catalyzing the reduction of molecular oxygen to water coupled to the translocation of protons across the mitochondrial inner membrane to drive ATP synthesis. The 3 largest subunits forming the catalytic core are encoded by mitochondrial DNA, while the other smaller subunits, including COX IV, are nuclear-encoded. Research studies have shown that deficiency in COX activity correlates with a number of human diseases (4). The COX IV antibody can be used effectively as a mitochondrial loading control in cell-based research assays. 1.Ostermeier, C. et al. (1996) Curr. Opin. Struct. Biol. 6, 460-466. 2.Capaldi, R.A. et al. (1983) Biochim. Biophys. Acta 726, 135-148. 3.Kadenbach, B. et al. (2000) Free Radic. Biol. Med. 29, 211-221. 4.Barrientos, A. et al. (2002) Gene 286, 53-63.
研究领域
癌症,细胞生物学,纤维化,代谢,神经科学
翻译后修饰
unmodified
制备和贮存
保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #85707.

数据库链接
Entrez-Gene ID
1327
UniProt ID
P13073

参考图片

Confocal immunofluorescent analysis of HeLa cells using COX IV (4D11-B3-E8) Mouse mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Western blot analysis of extracts from various cell lines using COX IV (4D11-B3-E8) Mouse mAb.

Immunoprecipitation of COX IV from HeLa cell extracts, using a non-specific mouse IgG control antibody (lane 2) or COX IV (4D11-B3-E8) Mouse mAb (lane 3). Lane 1 is 10% input. Western blot was performed using COX IV (4D11-B3-E8) Mouse mAb.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma using COX IV (4D11-B3-E8) Mouse mAb.

声明 :本官网所有报价均为常温或者蓝冰运输价格,如有产品需要干冰运输,需另外加收干冰运输费。
货号:
11967S
一键复制
询价
供应商现货
20μl
100ul
选择数量
当前规格1件起购 
配送至
预计5-6个工作日送达,快递: 免运费,若需干冰额外收费