




Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human COX IV protein.


Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:100 |
Immunohistochemistry (Paraffin) | 1:50 |
Immunofluorescence (Immunocytochemistry) | 1:200 |





Specificity/Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey




Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #85707.


参考图片
Confocal immunofluorescent analysis of HeLa cells using COX IV (4D11-B3-E8) Mouse mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Western blot analysis of extracts from various cell lines using COX IV (4D11-B3-E8) Mouse mAb.
Immunoprecipitation of COX IV from HeLa cell extracts, using a non-specific mouse IgG control antibody (lane 2) or COX IV (4D11-B3-E8) Mouse mAb (lane 3). Lane 1 is 10% input. Western blot was performed using COX IV (4D11-B3-E8) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using COX IV (4D11-B3-E8) Mouse mAb.