CR1/CD35 (E8B1Z) Rabbit mAb

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CR1/CD35 (E8B1Z) Rabbit mAb

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

来源纯化

Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human CR1/CD35 protein.

宿主

Rabbit

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunohistochemistry (Paraffin)	1:800 - 1:3200

同种型

Rabbit IgG

分子量

220

应用

目标/特异性

Specificity/Sensitivity

CR1/CD35 (E8B1Z) Rabbit mAb recognizes endogenous levels of total CR1/CD35 protein. Non-specific staining was observed in skeletal muscle by immunohistochemistry.

Species Reactivity:

Human

敏感性

Endogenous

背景

Complement receptor type 1 (CR1/CD35) is a type I transmembrane glycoprotein that is expressed on the surface of B cells, neutrophils, monocytes, and renal podocytes (1,2). As a component of the host innate immune system, CR1/CD35 expressed on neutrophils and monocytes binds to ligands coated with the complement opsonins, C3b and C4b, which facilitates phagocytosis and production of proinflammatory cytokines (3,4). CR1/CD35 also participates in negative regulation of the complement cascade through its ability to promote dissociation of C3 and C5 convertases (4,5) and by serving as one of multiple cofactors for factor-I-mediated cleavage and inactivation of C3b and C4b (6). 1.Fearon, D.T. (1980) J Exp Med 152, 20-30. 2.Tedder, T.F. et al. (1983) J Immunol 130, 1668-73. 3.Krych-Goldberg, M. and Atkinson, J.P. (2001) Immunol Rev 180, 112-22. 4.Bacle, F. et al. (1990) J Immunol 144, 147-52. 5.Hourcade, D.E. et al. (2002) J Biol Chem 277, 1107-12. 6.Masaki, T. et al. (1992) J Biochem 111, 573-8.

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

数据库链接

Entrez-Gene ID

1378

UniProt ID

P17927

参考图片

Immunohistochemical analysis of paraffin-embedded TF-1 cell pellet (left, positive) or K-562 cell pellet (right, negative) using CR1/CD35 (E8B1Z) Rabbit mAb.

Western blot analysis of extracts from various cell lines using CR1/CD35 (E8B1Z) Rabbit mAb (upper) or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower). Negative expression of CR1/CD35 protein in K-562 and NALM6 cells is consistent with the predicted expression pattern.

Western blot analysis of extracts from 293T cells, mock transfected (-) or transfected with a construct expressing Myc/DDK-tagged full-length human CR1/CD35 (hCR1/CD35-Myc/DDK; +), using CR1/CD35 (E8B1Z) Rabbit mAb (upper), DYKDDDDK Tag (D6W5B) Rabbit mAb #14793 (middle), or α-Actinin (D6F6) XP® Rabbit mAb #6487 (lower).

Immunohistochemical analysis of paraffin-embedded human Hodgkin lymphoma using CR1/CD35 (E8B1Z) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human tonsil using CR1/CD35 (E8B1Z) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded normal human kidney using CR1/CD35 (E8B1Z) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded Peyer's patch within normal human small intestine using CR1/CD35 (E8B1Z) Rabbit mAb.

Immunohistochemical analysis of paraffin-embedded human tonsil using CR1/CD35 (E8B1Z) Rabbit mAb (left) compared to concentration-matched Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (right).

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货号：

96615T

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询价

20μl

100ul

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