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Product Summary
The Parameter Creatinine assay is a 30 minute chemical analysis designed to measure Creatinine in urine.
Recovery
The recovery of Creatinine spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Urine (n=4) | 96 | 92-97 |
Linearity
To assess the linearity, nine urine samples were serially diluted with deionized or distilled water and assayed in two lots of kit reagents.
Scientific Data
Assay Procedure
Refer to the product Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 50 µL of Standard, control, or sample to each well.
- Add 100 µL of Alkaline Picrate Solution to each well.
- Incubate at room temperature for 25-35 minutes. Read at 490 nm.
50 µL Standard, Control, or Sample
100 µL Alkaline Picrate Solution
Creatinine Parameter Assay Kit Summary
Format
96-well strip plate
Assay Length
< 1 hour
Sample Type & Volume Required Per Well
Urine (10 uL)
Sensitivity
0.07 mg/dL
Assay Range
0.3 - 20 mg/dL (Urine)
Specificity
Measures Creatinine levels in urine
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity observed with 1 or more species tested.
Interference
Interference observed with other substances.
背景
别名
Creatinine
背景
Background: Creatinine
Creatinine is a non-enzymatic metabolite of phospho-Creatine, a molecule used primarily by muscle tissue as a store for high-energy phosphate that can be utilized for the production of ATP. Creatinine diffuses into the blood and is excreted by the kidneys at a relatively constant rate. Creatinine levels in the urine are used as a measurement of renal function.
Alternate Names:
Creatinine
研究领域
制备和贮存
保存方式
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Storage conditions vary by component. See product insert for storage instructions.
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