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Rabbit anti-CRYAA Polyclonal Antibody
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Rabbit anti-CRYAA Polyclonal Antibody
品牌： Absin
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对比
咨询分子量：
Detects a band of approximately 23kD (Predicted molecular weight: 19kD)
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
反应种属：
Human,Mouse,Rat
产品介绍
产品介绍
产品信息
荧光素标记
纯化方式
Affinity purification
宿主
Rabbit
免疫原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-173 of human CRYAA (NP_000385.1).
同种型
IgG
分子量
Detects a band of approximately 23kD (Predicted molecular weight: 19kD)
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
克隆类别
Polyclonal Antibody
应用
实验应用
WB:1:2000-1:6000, IF/ICC:1:50-1:200, ELISA:Recommended starting concentration is 1 μg/mL.
反应种属
Human,Mouse,Rat
阳性对照
Mouse kidney,Mouse liver,Mouse heart
背景
别名
CRYAA抗体
翻译后修饰
Unmodified
细胞定位
Cytoplasm,Nucleus
制备和贮存
存储溶液
PBS with 0.02% sodium azide,50% glycerol,pH7.3.
保存方式
Store at -20℃. Avoid freeze / thaw cycles.
参考图片
Western blot analysis of lysates from HepG2 cells, using CRYAA Rabbit pAb at 1:5000 dilution.|Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.|Lysates/proteins: 25μg per lane.|Blocking buffer: 3% nonfat dry milk in TBST.|Detection: ECL Basic Kit .|Exposure time: 180s.
Immunofluorescence analysis of paraffin-embedded Rat Eye using CRYAA Rabbit pAb at dilution of 1:200 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
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