Mouse anti-CTNNB1 Monoclonal Antibody(C-term)

Unlabeled

Mouse

IgG1

Predicted molecular weight: 85 kDa

Monoclonal Antibody

WB: 1:4000, IF: 1:25

Human, Mouse, Rat

This CTNNB1 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 687-721 amino acids from the C-terminal region of human CTNNB1.

CTNNB1抗体

Unmodified

Cytoplasm. Nucleus. Cytoplasm, cytoskeleton {ECO:0000250|UniProtKB:B6V8E6}. Cell junction, adherens junction {ECO:0000250|UniProtKB:Q02248}. Cell junction {ECO:0000250|UniProtKB:B6V8E6}. Cell membrane. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole Cell junction, synapse {ECO:0000250|UniProtKB:Q02248}. Cytoplasm, cytoskeleton, cilium basal body {ECO:0000250|UniProtKB:Q02248} Note=Colocalized with RAPGEF2 and TJP1 at cell-cell contacts (By similarity). Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization. The majority of beta-catenin is localized to the cell membrane. In interphase, colocalizes with CROCC between CEP250 puncta at the proximal end of centrioles, and this localization is dependent on CROCC and CEP250. In mitosis, when NEK2 activity increases, it localizes to centrosomes at spindle poles independent of CROCC. Colocalizes with CDK5 in the cell-cell contacts and plasma membrane of undifferentiated and differentiated neuroblastoma cells Interaction with FAM53B promotes translocation to the nucleus (PubMed:25183871). {ECO:0000250|UniProtKB:B6V8E6, ECO:0000269|PubMed:25183871}

Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.