BD Pharmingen™ APC Rat anti-Mouse CD184

CD184

Rat IgG2b, κ

GST-NCXCR4 fusion protein

The 2B11/CXCR4 monoclonal antibody specifically reacts with mouse CD184, which is also known as CXC chemokine receptor, CXCR4.  CXCR4 (previously known as Fusin and LESTR), a seven-transmembrane, G-protein-coupled receptor, is the specific receptor for CXC chemokines, SDF-1/CXCL12.  Mouse CXCR4 shows 91% homology at amino acid level with human CXCR4. CXCR4 is widely expressed by hematopoietic and non-hematopoietic cell types including neutrophils, monocytes, T cells, B cells, CD34-positive progenitor cells, endothelial cells, neurons and astrocytes.  In the thymus CXCR4 is restricted to CD4+CD8+ cells, while in the spleen, predominant expression is found on B lymphocytes.  Human CXCR4 is used by T-tropic HIV-1 as a co-receptor for viral entry.  The mouse CXCR4 gene has been mapped to chromosome 1.

2B11/CXCR4 The 2B11/CXCR4 monoclonal antibody specifically reacts with mouse CD184, which is also known as CXC chemokine receptor, CXCR4.  CXCR4 (previously known as Fusin and LESTR), a seven-transmembrane, G-protein-coupled receptor, is the specific receptor for CXC chemokines, SDF-1/CXCL12.  Mouse CXCR4 shows 91% homology at amino acid level with human CXCR4. CXCR4 is widely expressed by hematopoietic and non-hematopoietic cell types including neutrophils, monocytes, T cells, B cells, CD34-positive progenitor cells, endothelial cells, neurons and astrocytes.  In the thymus CXCR4 is restricted to CD4+CD8+ cells, while in the spleen, predominant expression is found on B lymphocytes.  Human CXCR4 is used by T-tropic HIV-1 as a co-receptor for viral entry.  The mouse CXCR4 gene has been mapped to chromosome 1.

Rat IgG2b, κ

克隆 2B11/CXCR4 (RUO)

0.2 mg/ml

Flow cytometry (Routinely Tested)

Mouse (QC Testing)

CD184 (CXCR4)

CXCR4, C-X-C chemokine receptor type 4; Fusin; LESTR; PB-CKR; Sdf1r

Aqueous buffered solution containing ≤0.09% sodium azide.

研发参考(10) 1. Bleul CC, Farzan M, Choe H, et al. The lymphocyte chemoattractant SDF-1 is a ligand for LESTR/fusin and blocks HIV-1 entry. Nature. 1996; 382(6594):829-833. (Biology). 2. Bleul CC, Wu L, Hoxie JA, Springer TA, Mackay CR. The HIV coreceptors CXCR4 and CCR5 are differentially expressed and regulated on human T lymphocytes.. Proc Natl Acad Sci U S A. 1997; 94(5):1925-1930. (Biology). 3. Feng Y, Broder CC, Kennedy PE, Berger EA. HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor. Science. 1996; 272(5263):872-877. (Biology). 4. Forster R, Kremmer E, Schubel A, et al. Intracellular and surface expression of the HIV-1 coreceptor CXCR4/fusin on various leukocyte subsets: rapid internalization and recycling upon activation. J Immunol. 1998; 160(3):1522-1531. (Immunogen). 5. Gupta SK, Lysko PG, Pillarisetti K, Ohlstein E, Stadel JM. Chemokine receptors in human endothelial cells. Functional expression of CXCR4 and its transcriptional regulation by inflammatory cytokines. J Biol Chem. 1998; 273(7):4282-4287. (Biology). 6. Heesen M, Berman MA, Benson JD, Gerard C, Dorf ME. Cloning of the mouse fusin gene, homologue to a human HIV-1 co-factor. J Immunol. 1996; 157(12):5455-5460. (Biology). 7. Hesselgesser J, Halks-Miller M, DelVecchio V, et al. CD4-independent association between HIV-1 gp120 and CXCR4: functional chemokine receptors are expressed in human neurons. Curr Biol. 1997; 7(2):112-121. (Biology). 8. Loetscher M, Geiser T, O'Reilly T, Zwahlen R, Baggiolini M, Moser B. Cloning of a human seven-transmembrane domain receptor, LESTR, that is highly expressed in leukocytes. J Biol Chem. 1994; 269(1):232-237. (Biology). 9. Oberlin E, Amara A, Bachelerie F, et al. The CXC chemokine SDF-1 is the ligand for LESTR/fusin and prevents infection by T-cell-line-adapted HIV-1. Nature. 1996; 382(6594):833-835. (Biology). 10. Schabath R, Muller G, Schubel A, Kremmer E, Lipp M, Forster R. The murine chemokine receptor CXCR4 is tightly regulated during T cell development and activation. J Leukoc Biol. 1999; 66(6):996-1004. (Biology).

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