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对比
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Recovery
The recovery of natural BLC/BCA-1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 98 | 87-110 |
| EDTA Plasma (n=4) | 99 | 92-107 |
| Heparin Plasma (n=4) | 101 | 92-115 |
| Serum (n=4) | 99 | 86-108 |
Linearity
Scientific Data
View LargerHuman CXCL13/BLC/BCA-1 Quantikine ELISA Kit Correlation between European League Against Rheumatism Sjögren’s Syndrome Disease Activity Index (ESSDAI) scores and concentrations of nine screened serum proteins analyzed by ELISA in the validation cohort of patients with primary Sjögren’s syndrome (n = 58). The nine proteins were CXCL13, TNF receptor 2 (TNF-R2), CD48, B-cell activating factor (BAFF), programmed cell death protein 1 ligand 2 (PD-L2), lymphocyte activation gene-3 (LAG-3), beta 2-microglobulin ( beta 2MG), ephrin type-B receptor 2 (EPHB2), and CD163. Pearson’s correlation test was used. The correlation coefficients (r) and P values are shown in the table. P <0.05 was considered significant Image collected and cropped by CiteAb from the following open publication (https://arthritis-research.biomedcentral.com/articles/10.1186/s13075-016-1006-1), licensed under a CC-BY license. Not internally tested by R&D Systems.
Assay Procedure
Refer to the product- Prepare all reagents, standard dilutions, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 100 µL of Assay Diluent to each well.
- Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
- Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
- Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
- Aspirate and wash 4 times.
- Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
- Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Human CXCL13/BLC/BCA-1 Quantikine ELISA Kit Summary
Background: CXCL13/BLC/BCA-1
CXCL13/BLC/BCA-1 is a constitutively expressed chemokine that plays an important role in B and T cell homing. It is expressed by salivary gland epithelium, dendritic cells, osteoclasts, and peritoneal macrophages. It can form homodimers or heterodimers with FGF basic, and it signals through CXCR3 or CXCR5. CXCL13 induces the migration of naïve B cells and a subset of memory T cells to lymphoid tissue. It also promotes B1 cell migration into the omentum and peritoneum. In the fetus, CXCL13 attracts CD4+ CD3- IL-7 R alpha+ hematopoietic cells to sites of future Peyer’s patch development.