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Monoclonal antibody is produced by immunizing animals with recombinant rat cytochrome c protein.
Product Usage Information
| Application | Dilution |
|---|---|
| Immunoprecipitation | 1:100 |
| Immunofluorescence (Immunocytochemistry) | 1:300 |
| Flow Cytometry (Fixed/Permeabilized) | 1:200 - 1:400 |
Specificity/Sensitivity
Species Reactivity:
Human, Mouse, Rat
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
参考图片
Flow cytometric analysis of 293T cells using Cytochrome C (6H2.B4) Mouse mAb (solid line) or a concentration matched Mouse (G3A1) mAb IgG Isotype Control #5415 (dashed lines). Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody.
Immunoprecipitation of cytochrome c from RD cell extracts using Mouse (G3A1) mAb IgG1 Isotype Control #5415 (lane 2) or Cytochrome c (6H2.B4) Mouse mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Cytochrome c (D18C7) Rabbit mAb #11940.
Confocal immunofluorescent analysis of HeLa cells, untreated (left) or treated with Staurosporine (1 μM) #9953 and Z-VAD (50 μM) for 3 hours (right), using Cytochrome c (6H2.B4) Mouse mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).
Flow cytometric analysis of NIH/3T3 cells using Cytochrome C (6H2.B4) Mouse mAb (solid line) or a concentration matched Mouse (G3A1) mAb IgG Isotype Control #5415 (dashed lines). Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 488 Conjugate) #4408 was used as a secondary antibody.