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Cytochrome c (D18C7) Rabbit mAb
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Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro72 of human cytochrome c protein.
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
Specificity/Sensitivity
Species Reactivity:
Human, Mouse, Rat, Monkey
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #36974.
参考图片
Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle using Cytochrome c (D18C7) Rabbit mAb.
Western blot analysis of extracts from various cell lines using Cytochrome c (D18C7) Rabbit mAb.
Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Staurosporine #9953 (1 μM, 3 hr; +). Cells were fractionated into whole cell lysate (WCL), cytoplasm (Cyto), membrane (Mem), and cytoskeletal/nucleus (Nuc). Membrane fraction includes mitochondria.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Cytochrome c (D18C7) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).