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Cytochrome c (D18C7) Rabbit mAb
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Cytochrome c (D18C7) Rabbit mAb

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品牌: CST
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分子量:
14
反应种属:
Human,Mouse,Rat,Monkey,
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产品介绍
产品介绍
产品信息
荧光素标记
抗原名称
Cytochrome c
来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro72 of human cytochrome c protein.

宿主
Rabbit
商品描述

Product Usage Information

ApplicationDilution
Western Blotting1:1000
Immunohistochemistry (Paraffin)1:50 - 1:200
同种型
Rabbit IgG
分子量
14
内毒素水平
牛 , 猪
研究领域
癌症,细胞生物学,纤维化,神经科学,
应用
反应种属
Human,Mouse,Rat,Monkey,
预测反应种属
Bovine,Pig,
目标/特异性

Specificity/Sensitivity

Cytochrome c (D18C7) Rabbit mAb recognizes endogenous levels of total cytochrome c protein.

Species Reactivity:

Human, Mouse, Rat, Monkey

敏感性
Endogenous
背景
背景
Cytochrome c is a well conserved electron-transport protein and is part of the respiratory chain localized to mitochondrial intermembrane space (1). Upon apoptotic stimulation, cytochrome c released from mitochondria associates with procaspase-9 (47 kDa)/Apaf 1. This complex processes caspase-9 from inactive proenzyme to its active form (2). This event further triggers caspase-3 activation and eventually leads to apoptosis (3). 1.Schagger H.H. et al. (2002) Biochem. Biophys. Acta. 1555, 154-159. 2.Li, P. et al. (1997) Cell 91, 479-489. 3.Liu, X. et al. (1996) Cell 86, 147-157.
研究领域
癌症,细胞生物学,纤维化,神经科学,
翻译后修饰
unmodified
制备和贮存
保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

For a carrier free (BSA and azide free) version of this product see product #36974.

数据库链接
Entrez-Gene ID
54205
UniProt ID
P99999

参考图片

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle using Cytochrome c (D18C7) Rabbit mAb.

Western blot analysis of extracts from various cell lines using Cytochrome c (D18C7) Rabbit mAb.

Western blot analysis of extracts from HeLa cells, untreated (-) or treated with Staurosporine #9953 (1 μM, 3 hr; +). Cells were fractionated into whole cell lysate (WCL), cytoplasm (Cyto), membrane (Mem), and cytoskeletal/nucleus (Nuc). Membrane fraction includes mitochondria.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Cytochrome c (D18C7) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

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货号:
11940T
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