DAPI Solution 1.0mg

DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) is a nucleic acid stain that binds to A-T rich regions of DNA along the minor groove. DAPI is predominantly impermeant to live cells, allowing it to be used as a viability dye in unfixed cells to discriminate intact from membrane-compromised cells. Note, however, that high concentrations of the dye may still enter intact cells. Additionally, DAPI may be used to analyze DNA content in fixed cells, or as a nuclear counterstain in imaging or flow cytometry. When bound to double-stranded DNA, DAPI has an excitation wavelength maximum of 358 nm and an emission maximum of 461 nm. DAPI is also well excited by the violet laser line (eg, 405 nm). Note that DAPI also binds RNA. Under these conditions, DAPI emits maximally at 500 nm, but with less intensity than when bound to double-stranded DNA.

DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) is a nucleic acid stain that binds to A-T rich regions of DNA along the minor groove. DAPI is predominantly impermeant to live cells, allowing it to be used as a viability dye in unfixed cells to discriminate intact from membrane-compromised cells. Note, however, that high concentrations of the dye may still enter intact cells. Additionally, DAPI may be used to analyze DNA content in fixed cells, or as a nuclear counterstain in imaging or flow cytometry. When bound to double-stranded DNA, DAPI has an excitation wavelength maximum of 358 nm and an emission maximum of 461 nm. DAPI is also well excited by the violet laser line (eg, 405 nm). Note that DAPI also binds RNA. Under these conditions, DAPI emits maximally at 500 nm, but with less intensity than when bound to double-stranded DNA.

1.0 mg/ml

Bioimaging, Flow cytometry, Immunofluorescence (Tested During Development)

DAPI

研发参考(5) 1. Darzynkiewicz Z, Bruno S, Del Bino G, et al. Features of apoptotic cells measured by flow cytometry. Cytometry. 1992; 13(8):795-808. (Methodology: Flow cytometry). 2. Hotz MA, Gong J, Traganos F, and Darzynkiewicz Z. Flow cytometric detection of apoptosis: Comparison of the assays of in situ DNA degradation and chromatin changes. Cytometry. 1994; 15(3):237-244. (Methodology: Flow cytometry). 3. Otto F. DAPI staining of fixed cells for high-resolution flow cytometry of nuclear DNA. Methods Cell Biol. 1990; 33:105-110. (Methodology: Flow cytometry). 4. Shapiro HM. Practical flow cytometry, 3rd ed.. New York: Wiley-Liss; 1995:280-282. 5. Tanious FA, Veal JM, Buczak H, Ratmeyer LS, Wilson WD. DAPI (4',6-diamidino-2-phenylindole) binds differently to DNA and RNA: minor-groove binding at AT sites and intercalation at AU sites. Biochemistry. 1992; 31(12):3103-3112. (Biology).