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品牌: Usbiological
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Purified by Protein G affinity chromatography.
反应种属:
Rabbit
产品介绍
产品介绍
产品信息
抗原来源
Human

宿主
Rabbit

免疫原
Recombinant protein corresponding to aa140-259 from human lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial protein.

产品用途
Suitable for use in ELISA and Western Blot. Other applications not tested.

简单描述
The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components.

同种型
IgG

克隆类别
Polyclonal

纯度
Purified by Protein G affinity chromatography.

形式
Supplied as a liquid in 0.01M PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.

应用
实验应用
E WB

推荐用量
Western Blot: 1:500-1:5000 Optimal dilutions to be determined by the researcher.

反应种属
Rabbit

交叉反应性
Hu Mo

目标/特异性
Recognizes human DBT. Species Crossreactivity: mouse

稀释度
Western Blot: 1:500-1:5000 Optimal dilutions to be determined by the researcher.
背景
别名
Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial (EC 2.3.1.168) (52kD mitochondrial autoantigen of primary biliary cirrhosis) (Branched chain 2-oxo-acid dehydrogenase complex component E2) (BCOADC-E2) (Branched-chain alpha-keto acid dehydrogenase complex component E2) (BCKAD-E2) (BCKADE2) (Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex) (Dihydrolipoamide branched chain transacylase) (Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase), DBT, BCATE2

制备和贮存
保存方式
Storage_Temp:-20°C
Storage_and_Stability:May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
数据库链接
UniProt ID
P11182

SwissProt
P11182

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