BD Stemflow™ Human Definitive and Pancreatic Endoderm Analysis Kit

Def

The BD Stemflow™ Human Definitive and Pancreatic Endoderm Analysis Kit contains a combination of mouse monoclonal antibody conjugates for the analysis of definitive and pancreatic endodermal differentiation cultures. The antibody specificities that are provided in this kit and the cell populations they can identify are listed in the table below. The antibody components of this kit can be used individually or in combinations of up to four.  Additional antibody formats that can enable more complex panels are available at www.bdbiosciences.com. The kit also includes BD Cytofix™ Fixation Buffer and BD Phosflow™ Perm Buffer III. Kit Components Component          Description                                                                         Size                   Vol. Per Test          Storage Buffer 51-9008006          Alexa Fluor® 488 Mouse Anti-Human Pax-6          25 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤0.09% sodium azide 51-9008007          PE Mouse Anti-PDX-1                                                       25 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤0.09% sodium azide 51-9008008          PE Mouse Anti-Human FoxA2                                     25 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤0.09% sodium azide 51-9007227          PerCP-Cy™5.5 Mouse Anti-Sox2                                     25 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤0.09% sodium azide 51-9008009          PerCP-Cy™5.5 Mouse Anti-Human Sox17                   25 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤0.09% sodium azide 51-9008010          Alexa Fluor® 647 Mouse Anti-Human Nanog          25 Test                   5 µl                   Aqueous buffered solution containing                                                                                                                                                                   BSA and ≤0.09% sodium azide 51-9006607          Perm Buffer III                                                                50 ml                                     51-9006276          Fixation Buffer                                                                50 ml Specificity                   Clone                            Cell Population Identified Nanog                            N31-335                            Pluripotent stem cells Sox2                            O30-678                            Pluripotent stem cells, ectodermal contaminants Sox17                            P7-969                            Definite endoderm FoxA2                            N17-280                            Definitive endoderm Pax6                            O30-1330                   Pancreatic endoderm, ectodermal contaminants Pdx1                            658A5                            Pancreatic endoderm

(RUO)

Intracellular staining (flow cytometry) (Routinely Tested)

Human (QC Testing)

Nanog, Sox2, Sox17, FoxA2, Pax-6, PDX-1

研发参考(5) 1. D'Amour KA, Agulnick AD, Eliazer S, Kelly OG, Kroon E, Baetge EE. Efficient differentiation of human embryonic stem cells to definitive endoderm.. Nat Biotechnol. 2005; 23(12):1534-41. (Methodology: Cell differentiation). 2. D'Amour KA, Bang AG, Eliazer S, et al . Production of pancreatic hormone-expressing endocrine cells from human embryonic stem cells. Nat Biotechnol. 2006; 24(12):1481-1483. (Methodology: Cell differentiation). 3. Kelly OG, Chan MY, Martinson LA, et al. Cell-surface markers for the isolation of pancreatic cell types derived from human embryonic stem cells. Nat Biotechnol. 2011; 29(8):750-756. (Methodology: Cell separation). 4. Kroon E, Martinson LA, Kadoya K, Bang AG, et al. Pancreatic endoderm derived from human embryonic stem cells generates glucose-responsive insulin-secreting cells in vivo. Nat Biotechnol. 2008; 26(4):443-452. (Biology: Cell differentiation). 5. Wang P, Rodriguez RT, Wang J, Ghodasara A, Kim SK. Targeting SOX17 in Human Embryonic Stem Cells Creates Unique Strategies for Isolating and Analyzing Developing Endoderm. Cell Stem Cell. 2011; 8:335-346. (Biology).