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产品介绍
产品信息
荧光素标记
纯化方式
Protein A or G purified from hybridoma culture supernatant
抗原名称
dickkopf (Xenopus laevis) homolog 1， dickkopf homolog 1 (Xenopus laevis)， dickkopf related protein-1
宿主
Mouse IgG1
免疫原
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Dkk-1
Thr32-His266
Accession # O94907
Thr32-His266
Accession # O94907
克隆号
141119
Human Dkk-1 Antibody Summary
Species Reactivity
Human
Specificity
Detects human Dkk-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody shows approximately 50% cross‑reactivity with recombinant mouse (rm) Dkk-1 and no cross-reactivity with rmDkk-2, rhDkk-3, or rhDkk-4.
Source
Monoclonal Mouse IgG1 Clone # 141119
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Dkk-1
Thr32-His266
Accession # O94907
Thr32-His266
Accession # O94907
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Recommended Concentration
Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
应用
实验应用
Western Blot
背景
别名
dickkopf (Xenopus laevis) homolog 1,dickkopf homolog 1 (Xenopus laevis),dickkopf related protein-1,Dickkopf-1,dickkopf-related protein 1,Dkk1,Dkk-1,hDkk-1,SKdickkopf-1 like,Dickkopf-1
背景
Background: Dkk-1
Dkk-1 is a Dickkopf-related protein that inhibits Wnt signaling by binding the Wnt co-receptor LRP5/6. It also binds Kremen-1 and Kremen-2. Dkk-1 is important in embryonic development.
Long Name
Dickkopf-1
Entrez Gene IDs
22943 (Human); 13380 (Mouse); 102123735 (Cynomolgus Monkey)
Alternate Names
dickkopf (Xenopus laevis) homolog 1; dickkopf homolog 1 (Xenopus laevis); dickkopf related protein-1; Dickkopf-1; dickkopf-related protein 1; Dkk1; Dkk-1; hDkk-1; SKdickkopf-1 like
研究领域
制备和贮存
溶解方法
Reconstitute at 0.5 mg/mL in sterile PBS.
保存方式
Preparation and Storage
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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