DNA Purification Buffers and Spin Columns (ChIP, CUT&RUN, CUT&Tag)

This product is offered to enable DNA purification using spin columns with our CUT&RUN Assay Kit (#86652).

This product also is offered to conveniently provide additional buffer reagents when using our SimpleChIP® (#9002, #9003) and SimpleChIP® Plus (#9004, #9005) Enzymatic Chromatin IP Kits, SimpleChIP® Sonication Chromatin IP Kit (#56383), and CUT&Tag Assay Kit (#77552). These kits provide all the reagents required for performing the respective assays, however there are instances where extra buffers are desired.

The DNA purification columns combine the convenience of spin-column technology with the selective binding properties of a uniquely designed silica membrane that allows for efficient recovery of DNA and removal of protein contaminants without the need for phenol/chloroform extractions and ethanol precipitations. After DNA purification, the enrichment of particular DNA sequences can be analyzed by a variety of methods.

Product Usage Information

Use as directed in the respective SimpleChIP® Chromatin IP kit, the CUT&RUN Assay kit, and the CUT&Tag Assay kit protocols. Please note that if following the SimpleChIP® Chromatin protocol or the CUT&Tag protocol, there will be excess DNA Binding Buffer (#10007) to accommodate the CUT&RUN protocol.