Donkey Polyclonal Donkey F(ab‘)2 anti-Sheep IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed)

Multi-step

Donkey

Donkey F(ab')2 anti-Sheep IgG (H+L) Secondary Antibody [PE] (Pre-adsorbed) was produced by repeated immunization with sheep IgG whole molecule in donkey.

This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis. This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Sheep IgG and Sheep Serum

IgG

Polyclonal

Immunogen affinity purified

0.01% Sodium Azide

Polyclonal

Flow Cytometry 1:100 - 1:250Immunocytochemistry/ Immunofluorescence 1:100 - 1:250

ShSpecies Glossary

No reaction was observed against anti-Pepsin, anti-Donkey IgG Fc, or Chicken, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit or Rat Serum Proteins.

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 10 mg/ml BSA