DR3 (D5Q2R) Rabbit mAb

The tumor necrosis factor receptor family, which includes TNF-RI, Fas, DR3, DR4, DR5, and DR6, plays an important role in the regulation of apoptosis in various physiological systems (1,2). The receptors are activated by a family of cytokines that include TNF, FasL, and TNF-related apoptosis-inducing ligand (TRAIL). They are characterized by a highly conserved extracellular region containing cysteine-rich repeats and a conserved intracellular region of about 80 amino acids termed the death domain (DD). The DD is important for transducing the death signal by recruiting other DD containing adaptor proteins (FADD, TRADD, RIP) to the death-inducing signaling complex (DISC), resulting in activation of caspases.DR3/WSL-1/Apo-3/TRAMP/LARD is a TNFR family member containing the characteristic extracellular cysteine-repeats, transmembrane region, and an intracellular DD (3-7). DR3 is activated by its ligand Apo-3L/TWEAK to induce apoptosis and activation of NF-κB (8,9). Like TNF-R1, DR3 binds to the DD adaptor protein TRADD, which can then associate with other DD proteins like FADD and RIP as well as members of the TRAF family (3,4). Tissue expression of DR3 is very restricted, primarily seen on the surface of activated thymocytes and lymphocytes and plays an important role in thymocyte negative selection (3,4,10). Studies have also indicated an association with DR3 and rheumatoid arthritis (11,12). 1.Nagata, S. (1997) Cell 88, 355-65. 2.Thorburn, A. (2004) Cell Signal 16, 139-44. 3.Chinnaiyan, A.M. et al. (1996) Science 274, 990-2. 4.Kitson, J. et al. (1996) Nature 384, 372-5. 5.Marsters, S.A. et al. (1996) Curr Biol 6, 1669-76. 6.Bodmer, J.L. et al. (1997) Immunity 6, 79-88. 7.Screaton, G.R. et al. (1997) Proc Natl Acad Sci U S A 94, 4615-9. 8.Marsters, S.A. et al. (1998) Curr Biol 8, 525-8. 9.Kaptein, A. et al. (2000) FEBS Lett 485, 135-41. 10.Wang, E.C. et al. (2001) Mol Cell Biol 21, 3451-61. 11.Osawa, K. et al. (2004) Genes Immun 5, 439-43. 12.Borysenko, C.W. et al. (2005) Biochem Biophys Res Commun 328, 794-9.