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Product Usage Information
Please follow CST’s recommended IF and Flow protocols. For both applications, following secondary detection:
Flow Cytometry: Centrifuge cells and resuspend in 0.5 ml DRAQ5® diluted 1:500 (10 µM) in PBS or Antibody Dilution Buffer. Incubate for 5 min at room temperature in the dark before analyzing cells on flow cytometer.
Immunofluorescence: Rinse samples twice in PBS for five minutes each. Dilute DRAQ5® 1:1000 (5 µM) in PBS and incubate for 5 minutes at room temperature in the dark. Rinse samples once in PBS, coat coverslips with anti-fade reagent and examine immediately using appropriate excitation wavelength.
应用
实验应用
IF-P/IF-F/IF-IC/F
背景
背景
DRAQ5®, 1,5-bis{[2-(di-methylamino) ethyl]amino}-4, 8-dihydroxyanthracene-9,10-dione, is a cell permeable far-red fluorescent DNA dye that can be used in live or fixed cells. This dye can be used in combination with GFP or FITC labels. DRAQ5® has been used to examine cellular DNA in flow cytometry and fluorescent microscopy applications (1-3).
1.Smith, P.J. et al. (2000) Cytometry 40, 280-91.
2.Wiltshire, M. et al. (2000) Cytometry 39, 217-23.
3.Smith, P.J. et al. (1999) J Immunol Methods 229, 131-9.
翻译后修饰
unmodified
制备和贮存
保存方式
Store at 4°C protected from light.
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