BD Pharmingen™ DRAQ7™

品牌： BD Pharmingen

下载产品说明书

下载SDS

用小程序，查商品更便捷

对比

咨询

产品介绍

产品信息

简单描述

DRAQ7™ (Deep Red Anthraquinone 7) is a far-red fluorescent DNA dye. DRAQ7™ is cell impermeable and may be used to stain nucleic acids in fixed cells for cell cycle analysis by DNA content, nuclear visualization, or discrimination of nucleated cells from debris or enucleated cells. Because DRAQ7 is impermeable to intact cells, it may also be used as a viability dye. DRAQ7™ has an excitation wavelength maxima of 599/644 nm, but can also be suboptimally excited by the 488 nm wavelength laser. Its emission wavelength maximum is 678 nm, or 694 nm when intercalated with double-stranded DNA.

克隆号

(RUO)

应用

目标/特异性

DRAQ7

制备和贮存

存储溶液

Aqueous buffered solution containing proprietary ingredients.

文献

Development References(5) 1. Akagi J, Kordon M, Zhao H, et al. Real-time cell viability assays using a new anthracycline derivative DRAQ7®. Cytometry A. 2013; 83(2):227-234. (Methodology). 2. Edward R. Red/far-red fluorescing DNA-specific anthraquinones for nucl:cyto segmentation and viability reporting in cell-based assays. Methods Enzymol. 2012; 505:23-45. (Methodology). 3. Smith PJ, Blunt N, Wiltshire M, et al. Characteristics of a novel deep red/infrared fluorescent cell-permeant DNA probe, DRAQ5, in intact human cells analyzed by flow cytometry, confocal and multiphoton microscopy. Cytometry. 2000; 40(4):280-291. (Methodology). 4. Smith PJ, Wiltshire M, Davies S, et al. A novel cell permeant and far red-fluorescing DNA probe, DRAQ5, for blood cell discrimination by flow cytometry. J Immunol Methods. 1999; 229(1):131-139. (Methodology). 5. Smith PJ, Wiltshire M, Errington RJ. DRAQ5 Labeling of Nuclear DNA in Live and Fixed Cells. Curr Protoc Cytom. 7(7.25)(Methodology).

参考图片

Panel 1. Two-color flow cytometric analysis of Jurkat cell viability. Jurkat cells were treated with 5 µM Camptothecin (Right Plot) or DMSO vehicle (Left Plot) overnight. Cells were resuspended in Annexin V Binding Buffer (Cat. No. 556454) and stained with FITC Annexin V (Cat. No. 556419) and 1.25 µM DRAQ7™ (Cat. No. 564904). Camptothecin-treated cells show an increased frequency of apoptotic (Annexin V+ DRAQ7™-) and dead (Annexin V+ DRAQ7™+) cells. Analysis was performed using a BD LSRFortessa™ Cell Analyzer System. Panel 2. Flow cytometric analysis of HeLa cell DNA content. Cultured HeLa cells in log phase growth were harvested using Gibco® Cell Dissociation Buffer (Life Technologies), fixed, and permeabilized using the BD Pharmingen™ Transcription Factor Buffer Set (Cat. No. 562574/562725). Cells were resuspended in DPBS with 20 μM DRAQ7™ and analyzed using a low BD LSRFortessa™ cytometer flow rate. Histograms were deconvoluted by FlowJo™ software into G0/G1, S, and G2/M populations. Panel 3. Immunoflourescent staining of Alkaline Phosphatase on human embryonic stem (ES) cells. H9 human ES cells (WiCell, Madison, WI) passage 44 were cultured on mTeSR™1 medium (StemCell Technologies) and fixed with BD Cytofix™ fixation buffer (Cat. No. 554655). The fixed cells were stained with Alexa Fluor® 488 Mouse anti-Human Alkaline Phosphatase monoclonal antibody (pseudo-colored green, Cat. No. 561495). And 5 μM DRAQ7™ (pseudo-colored red) was used as a nuclear counterstain. The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ Software. DRAQ7™ was also tested in mouse (data not shown).

声明：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。

货号：

564904

一键复制

询价

1.0ml

选择数量

当前规格1件起购

配送至

预计3-4周送达，快递: 免运费，若需干冰额外收费

询价大包装询价