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产品介绍
产品介绍
产品信息
荧光素标记
纯化方式
Protein A or G purified from hybridoma culture supernatant
抗原名称
dishevelled 2 (homologous to Drosophila dsh)， dishevelled, dsh homolog 2 (Drosophila)， Dishevelled2，
宿主
Mouse IgG1
免疫原
E. coli-derived recombinant human Dishevelled-2
Leu78-Thr250
Accession # O14641
Leu78-Thr250
Accession # O14641
克隆号
373413
Human Dishevelled-2 Antibody Summary
Species Reactivity
Human
Specificity
Detects human Dishevelled‑2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) DVL-1 or rhDVL-3 is observed.
Source
Monoclonal Mouse IgG1 Clone # 373413
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Dishevelled-2
Leu78-Thr250
Accession # O14641
Leu78-Thr250
Accession # O14641
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Dishevelled‑2
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
应用
实验应用
Western Blot(1 µg/mL)
背景
别名
dishevelled 2 (homologous to Drosophila dsh),dishevelled, dsh homolog 2 (Drosophila),Dishevelled2,Dishevelled-2,DSH homolog 2,DVL2,segment polarity protein dishevelled homolog DVL-2
背景
Background: Dishevelled-2
Dishevelled-2 (DVL-2) is a 90‑95 kDa cytoplasmic phosphoprotein that is one of the three Drosophila dishevelled orthologs. Human DVL-2 is expressed in multiple tissues, participates in Wnt signal transduction and may mediate Wnt-dependent motility. DVL-2 is a modular protein containing DIX, PDZ and DEP linker regions that connect Wnt/Frizzled engagement to proteins involved in the inactivation of beta -catenin destruction in the Wnt canonical pathway. Human and mouse DVL-2 share 94% aa identity over the sequence used for immunization.
Entrez Gene IDs
1856 (Human); 13543 (Mouse); 303251 (Rat)
Alternate Names
dishevelled 2 (homologous to Drosophila dsh); dishevelled, dsh homolog 2 (Drosophila); Dishevelled2; Dishevelled-2; DSH homolog 2; DVL2; segment polarity protein dishevelled homolog DVL-2
研究领域
制备和贮存
溶解方法
Reconstitute at 0.5 mg/mL in sterile PBS.
保存方式
Preparation and Storage
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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