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DYKDDDDK Tag Antibody (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody)

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DYKDDDDK Tag Antibody (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody)

品牌： CST

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产品介绍

产品信息

荧光素标记

Unconjugated

抗原名称

DYKDDDDK Tag

来源纯化

Polyclonal antibodies are produced by immunizing animals with a synthetic DYKDDDDK peptide. Antibodies are purified by protein A and peptide affinity chromatography.

宿主

Rabbit

商品描述

Product Usage Information

Application	Dilution
Western Blotting	1:1000
Immunoprecipitation	1:50
Flow Cytometry (Fixed/Permeabilized)	1:200

应用

反应种属

All

目标/特异性

Specificity/Sensitivity

DYKDDDDK Tag Antibody detects exogenously expressed DYKDDDDK proteins in cells. The antibody recognizes the DYKDDDDK peptide (the same epitope recognized by Sigma-Aldrich Anti-FLAG M2 antibody) fused to either the amino- or carboxy-terminus of targeted proteins.The binding specificity of this antibody is NOT dependent on the presence of divalent metal cations.

Species Reactivity:

All Species Expected

敏感性

Transfected Only

背景

Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion (1). 1.Brizzard, B. L. et al. (1994) Biotechniques 16, 730-735.

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.

参考图片

Flow cytometric analysis of DYKDDDDK Tag Rabbit antibody in Cos cells transfected with a Myc-DDK-tagged SUMF1 construct and gated on transfected (green) or untransfected (blue) cells using Myc-Tag (9B11) Mouse mAb (Alexa Fluor® Conjugate) #2279.

Western blot analysis of extracts from COS or 293T cells, mock transfected (lane 1) or expressing DYKDDDDK-tagged p73 (lane2), DYKDDDDK-tagged FKHRL1 (lane 3) or truncated DYKDDDDK-tagged huntingtin (lane 4), using DYKDDDDK Tag Antibody.

Western blot analysis of recombinant human SYN3-MYC/DYKDDDDK at various concentrations, using DYKDDDDK Tag Antibody.

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货号：

2368S

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