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DYKDDDDK Tag (9A3) Mouse mAb (Binds to same epitope as Sigma-Aldrich Anti-FLAG M2 antibody)
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Monoclonal antibody is produced by immunizing animals with a synthetic DYKDDDDK peptide.
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
| Immunofluorescence (Immunocytochemistry) | 1:800 - 1:1600 |
| Flow Cytometry (Fixed/Permeabilized) | 1:400 - 1:1600 |
Specificity/Sensitivity
Species Reactivity:
All Species Expected
Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
For a carrier free (BSA and azide free) version of this product see product #47880.
参考图片
Flow cytometric analysis of 293T cells, mock-transfected (blue, negative) or transfected with a construct expressing DYKDDDDK Tag (green, positive), using DYKDDDDK Tag (9A3) Mouse mAb (solid lines) or concentration-matched Mouse (G3A1) mAb IgG1 IsotypeControl #5415 (dashed lines). Anti-mouse IgG (H+L), F(ab')2 Fragment (Alexa Fluor® 647 Conjugate) #4410 was used as a secondary antibody.
Western blot analysis of extracts from 293T cells, untransfected or transfected with either DYKDDDDK-tagged FoxG1, NPAS4, or Tyrosine Hydroxylase (TH), using DYKDDDDK Tag (9A3) Mouse mAb.
Western blot analysis of recombinant human SYN3-MYC/DYKDDDDK at various concentrations using, DYKDDDDK Tag (9A3) Mouse mAb.
Immunoprecipitation of PI3K-DYKDDDDK tag protein from 293 transfected cell extracts. Lane 1 is 10% input, lane 2 is Mouse (G3A1) mAb IgG1 Isotype Control #5415, and lane 3 is DYKDDDDK Tag (9A3) Mouse mAb. Western blot analysis was performed using DYKDDDDK Tag (D6W5B) Rabbit mAb #14793. Anti-rabbit IgG, HRP-linked Antibody #7074 was used as a secondary antibody.
Immunohistochemical analysis of paraffin-embedded 293T cell pellets, control (left) or ROS-DYKDDDDK Tag transfected (right), using DYKDDDDK Tag (9A3) Mouse mAb.
Confocal immunofluorescent analysis of HeLa cells transfected with a DYKDDDDK-tagged IRF3 construct using DYKDDDDK Tag (9A3) Mouse mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).