PERK (D11A8) Rabbit mAb

Protein kinase-like endoplasmic reticulum kinase (PERK) is an eIF2α kinase and transmembrane protein resident in the endoplasmic reticulum (ER) membrane that couples ER stress signals to translation inhibition (1-3). ER stress increases the activity of PERK, which then phosphorylates eIF2α to promote reduced translation. Research studies have demonstrated that PERK-deficient mice have defects in pancreatic β cells several weeks after birth, suggesting a role for PERK-mediated translational control in protecting secretory cells from ER stress (4). PERK activation during ER stress correlates with autophosphorylation of its cytoplasmic kinase domain (1-3). Phosphorylation of PERK at Thr980 serves as a marker for its activation status. 1.Harding, H. et al. (1999) Nature 397, 271-274. 2.Shi, Y. et al. (1998) Mol. Cell. Biol. 18, 7499-7509. 3.Harding, H. et al. (2000) Mol. Cell 5, 897-904. 4.Harding, H. et al. (2001) Mol. Cell 7, 1153-1163.