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产品介绍
产品介绍
产品信息
克隆号
225 (HB-8508)
克隆类别
单抗
Product Details
The 225 monoclonal antibody reacts with an epitope on the extracellular domain of human EGFR belongs to the ErbB family of receptors. EGFR signaling is activated upon binding one of its ligands including epidermal growth factor (EGF), transforming growth factor α (TGF α), Amphiregulin, and heparin binding EGF (HB-EGF). Upon activation, EGFR transitions from an inactive monomeric form to an active homodimer. This initiates several downstream signal transduction cascades including the MAPK, Akt and JNK pathways, leading to DNA synthesis and cell proliferation. EGFR overexpression or constitutive activation are associated with many cancers. For this reason, anti-EGFR monoclonal antibody mediated immunotherapies are currently being explored as cancer treatments. The 225 antibody has been reported to neutralize EGFR signaling.Specifications
| Isotype | Mouse IgG1 |
|---|---|
| Recommended Isotype Control(s) | InVivoMAb mouse IgG1 isotype control, unknown specificity |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Conjugation | This product is unconjugated. Conjugation is available via our Antibody Conjugation Services. |
| Immunogen | Purified EGFR from A431 cells |
| Reported Applications | in vitro EGFR blockade in vivo EGFR blockade in xenografts Western blot Functional assays |
| Formulation | PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin | <2EU/mg (<0.002EU/μg) Determined by LAL gel clotting assay |
| Purity | >95% Determined by SDS-PAGE |
| Sterility | 0.2 μM filtered |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein G |
| RRID | AB_2687801 |
| Molecular Weight | 150 kDa |
| Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
组成成分
IGG-免疫球蛋白G型抗体
应用
反应种属
human
背景
研究领域
Cancer Biology, Tumor Antigens
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