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产品介绍
产品介绍
产品信息
宿主
鼠
克隆号
528
克隆类别
单抗
Product Details
The 528 monoclonal antibody reacts with an epitope on the extracellular domain of human EGFR (epidermal growth factor receptor) also known as ErbB-1. EGFR is a 170 kDa cell-surface receptor and belongs to the ErbB family of receptors. EGFR signaling is activated upon binding one of its ligands including epidermal growth factor (EGF), transforming growth factor α (TGF α), Amphiregulin, and heparin binding-EGF (HB-EGF). Upon activation, EGFR transitions from an inactive monomeric form to an active homodimer. This initiates several downstream signal transduction cascades including the MAPK, Akt and JNK pathways, leading to DNA synthesis and cell proliferation. EGFR overexpression or constitutive activation are associated with many cancers. For this reason, anti-EGFR monoclonal antibody mediated immunotherapies are currently being explored as cancer treatments. The 528 antibody has been reported to block EGF binding to its receptor and inhibit A431 tumor formation in nude mice.Specifications
| Isotype | Mouse IgG2a |
|---|---|
| Recommended Isotype Control(s) | InVivoMAb mouse IgG2a isotype control, unknown specificity |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Conjugation | This product is unconjugated. Conjugation is available via our Antibody Conjugation Services. |
| Immunogen | Purified EGFR from A431 cells |
| Reported Applications | in vitro EGFR blockade in vivo EGFR blockade in xenografts Western blot Functional assays Immunoprecipitation Immunohistochemistry (paraffin) Immunofluorescence Flow cytometry |
| Formulation | PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin | <2EU/mg (<0.002EU/μg) Determined by LAL gel clotting assay |
| Purity | >95% Determined by SDS-PAGE |
| Sterility | 0.2 µm filtration |
| Production | Purified from cell culture supernatant in an animal-free facility |
| Purification | Protein A |
| RRID | AB_2687802 |
| Molecular Weight | 150 kDa |
| Storage | The antibody solution should be stored undiluted at 4°C, and protected from prolonged exposure to light. Do not freeze. |
组成成分
表皮生长因子受体蛋白抗体，磷酸盐缓冲液
应用
反应种属
human
背景
研究领域
Cancer Biology
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