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EGR1 (44D5) Rabbit mAb (PE Conjugate)

品牌： CST

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产品介绍

产品信息

荧光素标记

抗原名称

EGR1

来源纯化

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of the sequence of human EGR1.

宿主

Rabbit

简单描述

This Cell Signaling Technology antibody is conjugated to phycoerythrin (PE) and tested in-house for direct flow cytometry analysis in human cells. This antibody is expected to exhibit the same species cross-reactivity as the unconjugated EGR1 (44D5) Rabbit mAb #4154.

商品描述

Product Usage Information

Application	Dilution
Flow Cytometry (Fixed/Permeabilized)	1:50

同种型

Rabbit IgG

克隆号

44D5

内毒素水平

牛

研究领域

神经科学

应用

反应种属

Human,Mouse,Rat,

预测反应种属

Bovine

目标/特异性

Specificity/Sensitivity

EGR1 (44D5) Rabbit mAb (PE Conjugate) detects endogenous levels of total EGR1 protein.

Species Reactivity:

Human, Mouse, Rat

敏感性

Endogenous

背景

EGR family members are transcriptional factors that contain three repetitive zinc finger DNA binding domains which bind to EGR response elements (ER) to regulate target gene expression (1). The expression of EGR family members is induced by growth factors, with EGR1 expression being induced by NGF (1,2). Increased EGR1 expression activates transcription of other signaling molecules, including CDK5 and tyrosine hydroxylase, and exerts long term effects on neural cell growth and differentiation (2,3). 1.O'Donovan, K.J. et al. (1999) Trends Neurosci. 22, 167-173. 2.Harada, T. et al. (2001) Nat. Cell Biol. 3, 453-459. 3.Papanikolaou, N.A. and Sabban, E.L. (2000) J. Biol. Chem. 275, 26683-26689.

研究领域

神经科学

翻译后修饰

unmodified

制备和贮存

保存方式

Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/ml BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.

数据库链接

Entrez-Gene ID

1958

UniProt ID

P18146

参考图片

Flow cytometric analysis of Jurkat cells, untreated (blue) or treated with TPA #4174 (200 nM, 2 hours) and Ionomycin, Calcium Salt #9995 (3 µM, 2 hours) (green), using EGR1 (44D5) Rabbit mAb (PE Conjugate).

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货号：

25357S

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询价

100ul

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