The TU66 monoclonal antibody specifically recognizes human CD39 which is also known as Ectonucleoside triphosphate diphosphohydrolase 1 (NTPDase 1), Ecto-ATP diphosphohydrolase 1 (Ecto-ATPDase 1), or Ecto-apyrase. CD39 is an integral membrane glycoprotein with two transmembrane domains, N- and C-terminal cytoplasmic tails, and an extracellular region that contains the NTPDase 1 active site. CD39 is encoded by
ENTPD1
which belongs to the ectoenzyme family. CD39 is variably expressed on activated T cells and B cells, regulatory T cells (Treg), dendritic cells, Langerhans cells, NK cells, monocytes, macrophages, endothelial cells, and granulocytes. CD39 acts on extracellular nucleoside triphosphates and diphosphates including ATP and ADP that are hydrolyzed into AMP. Through cell surface CD73 (Ecto-5'-nucleotidase), regulatory T cells can act on extracellular AMP to generate immunosuppressive adenosine. CD39 is involved in the control of the extracellular pool of phosphorylated nucleosides, the suppression of inflammation and immunity, and the regulation of platelet activation.
商品描述
TU66
The TU66 monoclonal antibody specifically recognizes human CD39 which is also known as Ectonucleoside triphosphate diphosphohydrolase 1 (NTPDase 1), Ecto-ATP diphosphohydrolase 1 (Ecto-ATPDase 1), or Ecto-apyrase. CD39 is an integral membrane glycoprotein with two transmembrane domains, N- and C-terminal cytoplasmic tails, and an extracellular region that contains the NTPDase 1 active site. CD39 is encoded by
ENTPD1
which belongs to the ectoenzyme family. CD39 is variably expressed on activated T cells and B cells, regulatory T cells (Treg), dendritic cells, Langerhans cells, NK cells, monocytes, macrophages, endothelial cells, and granulocytes. CD39 acts on extracellular nucleoside triphosphates and diphosphates including ATP and ADP that are hydrolyzed into AMP. Through cell surface CD73 (Ecto-5'-nucleotidase), regulatory T cells can act on extracellular AMP to generate immunosuppressive adenosine. CD39 is involved in the control of the extracellular pool of phosphorylated nucleosides, the suppression of inflammation and immunity, and the regulation of platelet activation.
同种型
Mouse IgG2b, κ
克隆号
克隆 TU66 (also known as Tü 66, Tü66) (RUO)
产品详情
PE
R-Phycoerythrin (PE), is part of the BD family of Phycobiliprotein dyes. This fluorochrome is a multimeric fluorescent phycobiliprotein with excitation maximum (Ex Max) of 496 nm and 566 nm and an emission maximum (Em Max) at 576 nm. PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 575/26-nm bandpass filter). As PE is excited by multiple lasers, this can result in cross-laser excitation and fluorescence spillover on instruments with various combinations of Blue, Green, and Yellow-Green lasers. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
PE
Yellow-Green 488 nm, 532 nm, 561 nm
496 nm, 566 nm
576 nm
应用
实验应用
Flow cytometry (Routinely Tested)
推荐用量
20 µl
反应种属
Human (QC Testing)
目标/特异性
CD39 (ENTPD1)
背景
别名
ENTPD1; NTPDase-1; Ecto-ATPase 1; Ecto-ATPDase 1
制备和贮存
存储溶液
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
保存方式
Aqueous buffered solution containing BSA and ≤0.09% sodium azide.
文献
文献
研发参考(4)
1. Borsellino G, Kleinewietfeld M, Di Mitri D, et al. Expression of ectonucleotidase CD39 by Foxp3+ Treg cells: hydrolysis of extracellular ATP and immune suppression.. Blood. 2007. (Biology).
2. Duensing S, Kirshner H, Atzpodien J. CD39 as a novel marker of in vivo immune activation. Blood. 1994; 83(12):3826-3827. (Biology).
3. Knapp W. W. Knapp .. et al., ed. Leucocyte typing IV : white cell differentiation antigens. Oxford New York: Oxford University Press; 1989:1-1182.
4. Schlossman SF. Stuart F. Schlossman .. et al., ed. Leucocyte typing V : white cell differentiation antigens : proceedings of the fifth international workshop and conference held in Boston, USA, 3-7 November, 1993. Oxford: Oxford University Press; 1995.
数据库链接
Entrez-Gene ID
953
参考图片
Flow cytometric analysis of PE Mouse anti-Human CD39 on peripheral blood. Human peripheral blood was isolated by Ficoll gradient and stained simultaneously with PerCP CD4 (clone SK3, Cat. No. 340671), Alexa Fluor 647 CD127 (clone hIL7R-M21, Cat. No. 558598) and PE CD39 (clone TU66, Cat. No. 555464). The cells were then fixed and permeabilized using the Foxp3 buffer kit (Cat. No. 560098) and then stained with Alexa Fluor 488 Foxp3 (clone 259D, Cat. No. 560047).The dot plot (data not shown) was derived from gated events based on light scattering characteristics of lymphocytes and fluorescence characteristics of CD4 positive cells. Regulatory T cells were then identified by the CD127 versus Foxp3 staining pattern (right panel) and analyzed for CD39 expression (left panel). Flow cytometry was performed on a BD FACSCanto™ System.
Flow cytometric analysis of PE Mouse anti-Human CD39 on peripheral blood. Human peripheral blood was isolated by Ficoll gradient and stained simultaneously with PerCP CD4 (clone SK3, Cat. No. 340671), Alexa Fluor 647 CD127 (clone hIL7R-M21, Cat. No. 558598) and PE CD39 (clone TU66, Cat. No. 555464). The cells were then fixed and permeabilized using the Foxp3 buffer kit (Cat. No. 560098) and then stained with Alexa Fluor 488 Foxp3 (clone 259D, Cat. No. 560047).The dot plot (data not shown) was derived from gated events based on light scattering characteristics of lymphocytes and fluorescence characteristics of CD4 positive cells. Regulatory T cells were then identified by the CD127 versus Foxp3 staining pattern (right panel) and analyzed for CD39 expression (left panel). Flow cytometry was performed on a BD FACSCanto™ System.
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