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Epithelial-Mesenchymal Transition (EMT) Antibody Sampler Kit
Epithelial-Mesenchymal Transition (EMT) Antibody Sampler Kit
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Epithelial-Mesenchymal Transition (EMT) Antibody Sampler Kit

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    产品介绍
    产品信息
    抗原名称
    Epithelial-Mesenchymal Transition
    来源纯化
    Monoclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg526 of human N-cadherin protein, a synthetic peptide corresponding to residues near the carboxy terminus of human claudin-1 protein, a synthetic peptide corresponding to residues near the carboxy terminus of human ZO-1 protein, a recombinant human Snail protein, a recombinant human Slug protein, a synthetic peptide corresponding to residues surrounding Arg45 of human vimentin protein, a synthetic peptide corresponding to residues surrounding Pro780 of human E-cadherin, a synthetic peptide corresponding to residues surrounding Asp868 of human TCF8/ZEB1 protein, or a synthetic peptide corresponding to residues surrounding Pro714 of human ß-catenin protein.
    简单描述
    Antibody Sampler Kit for studying in the research area.
    应用
    目标/特异性

    Specificity/Sensitivity

    E-Cadherin (24E10) Rabbit mAb detects endogenous levels of total E-cadherin protein. The antibody does not cross-react with related family members, such as N-cadherin. N-Cadherin (D4R1H) XP® Rabbit mAb recognizes endogenous levels of total N-cadherin protein. Claudin-1 (D5H1D) XP® Rabbit mAb recognizes endogenous levels of total claudin-1 protein. ZO-1 (D7D12) Rabbit mAb detects endogenous levels of total ZO-1 protein. Vimentin (D21H3) XP® Rabbit mAb detects endogenous levels of total vimentin protein. Snail (C15D3) Rabbit mAb detects endogenous levels of total Snail protein. Slug (C19G7) Rabbit mAb detects endogenous levels of total Slug protein. TCF8/ZEB1 (D80D3) Rabbit mAb detects endogenous levels of total TCF8/ZEB1 protein. ß-Catenin (D10A8) XP® Rabbit mAb detects endogenous levels of total ß-catenin protein.

    背景
    背景
    上皮-间质细胞转换 (EMT) 是发育期间的重要过程，在此过程中上皮细胞获得间质成纤维细胞样特性，表现出胞内黏附减少和运动性增加。这是正常胚胎发育的关键特征，恶性上皮细胞肿瘤会利用此特征进行肿瘤扩散 (1-3)。这一严格调控的过程与大量细胞和分子活动相关。EMT 依赖于细胞黏附分子表达的减少。钙粘蛋白介导钙依赖性细胞间黏附，并在正常组织发育中起关键作用 (4)。E-钙粘蛋白被认为是许多上皮癌细胞侵袭和生长的活性抑制蛋白 (4-6)。近期研究表明，除了 E 钙粘蛋白丢失外，癌细胞的 N-钙粘蛋白表达上调。钙粘蛋白表达变化称为“钙粘蛋白转换”，E-钙粘蛋白表达下调是 EMT 的一个标志 (1)。紧密连接或紧密闭合蛋白可在上皮细胞和内皮细胞之间形成一个连续的液体屏障。紧密连接可调节细胞旁通透性并维持细胞极性，阻断顶端和基底外侧细胞表面之间跨膜蛋白的运动。紧密连接由密封蛋白和闭合蛋白组成，并可与细胞骨架连接 (7,8)。封闭区蛋白 ZO-1、2 和 3（也称 TJP 1、2 和 3）是外周膜接头蛋白，可将闭合蛋白和密封蛋白等连接跨膜蛋白结合到肌动蛋白细胞骨架 (9)。ZO-1 和 -2 对紧密连接形成和功能不可或缺 (10,11)；ZO-1 和密封蛋白突变会诱导 EMT (12)。波形蛋白是间叶细胞的中间纤维，见于早期发育阶段。胞外刺激诱导的波形蛋白动态结构变化和空间重组，有助于协调不同的信号转导通路 (13)。β-catenin是 Wnt 信号转导通路的关键下游效应子 (14)，它与脊椎动物的两个主要生物学过程有关：早期胚胎发育（15）和肿瘤发生（16）。 β-catenin 也可激活 Slug。Slug (SNAI2) 是一种广泛表达的转录阻遏蛋白，也是锌指转录因子 Snail 家族的一员 (17)。与相关 Snail 蛋白一样，Slug 结合 E-钙粘蛋白启动子区来抑制发育期间的转录(18)。Slug 结合整合素启动子序列后，会抑制整合素表达、导致细胞黏附减少 (19)。在胚胎发育的 EMT 期间，E-钙粘蛋白表达会发生下调 (20)。ZEB 家族蛋白是包含锌指和同源盒结构域的转录因子。E-钙粘蛋白是 ZEB 蛋白所抑制的一个靶标蛋白 (1)。 Aigner, K. et al. (2007) Oncogene 26, 6979-88. Peinado, H. et al. (2007) Nat Rev Cancer 7, 415-28. Moreno-Bueno, G. et al. (2008) Oncogene 27, 6958-69. Wheelock, M.J. and Johnson, K.R. (2003) Annu Rev Cell Dev Biol 19, 207-35. Christofori, G. (2003) EMBO J 22, 2318-23. Hazan, R.B. et al. (2004) Ann N Y Acad Sci 1014, 155-63. Shin, K. et al. (2006) Annu Rev Cell Dev Biol 22, 207-35. Oliveira, S.S. and Morgado-Díaz, J.A. (2007) Cell Mol Life Sci 64, 17-28. Matter, K. and Balda, M.S. (2007) J Cell Sci 120, 1505-11. Hernandez, S. et al. (2007) Exp Cell Res 313, 1533-47. Umeda, K. et al. (2006) Cell 126, 741-54. Reichert, M. et al. (2000) J Biol Chem 275, 9492-500. Helfand, B.T. et al. (2004) J Cell Sci 117, 133-41. Cadigan, K.M. and Nusse, R. (1997) Genes Dev 11, 3286-305. Wodarz, A. and Nusse, R. (1998) Annu Rev Cell Dev Biol 14, 59-88. Polakis, P. (1999) Curr Opin Genet Dev 9, 15-21. Inukai, T. et al. (1999) Mol Cell 4, 343-52. Bolós, V. et al. (2003) J Cell Sci 116, 499-511. Turner, F.E. et al. (2006) J Biol Chem 281, 21321-31. Barrallo-Gimeno, A. and Nieto, M.A. (2005) Development 132, 3151-61.

    参考图片

    Western blot analysis of extracts from various cell lines using β-Catenin (D10A8) XP® Rabbit mAb.

    Immunohistochemical analysis of frozen mouse colon using β-Catenin (D10A8) XP® Rabbit mAb.

    After the primary antibody is bound to the target protein, a complex with HRP-linked secondary antibody is formed. The LumiGLO* is added and emits light during enzyme catalyzed decomposition.

    Western blot analysis of extracts from A431, MCF-7, C2C12, HUVEC, BAEC, C6 and H-4-II-E cells, using N-cadherin Antibody.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using E-Cadherin (24E10) Rabbit mAb in the presence of control peptide (left) or E-Cadherin Blocking Peptide #1050 (right).

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma, using E-Cadherin (24E10) Rabbit mAb.

    Western blot analysis of extracts from various cell lines, using E-Cadherin (24E10) Rabbit mAb.

    Immunohistochemical analysis of paraffin-embedded human metastatic adenocarcinoma in lymph node, using E-Cadherin (24E10) Rabbit mAb.

    Confocal immunofluorescent images of MCF7 cells using E-Cadherin (24E10) Rabbit mAb (green, left) compared to an isotype control (right). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).

    Immunohistochemical analysis of frozen HCC827 xenograft, showing membrane and cytoplasmic localization using E-Cadherin (24E10) Rabbit mAb.

    Western blot analysis of extracts from A431 and ZR-75 cells using Claudin-1 Antibody.

    Immunoprecipitation of claudin-1 from A431 cell extract using Claudin-1 Antibody. Western blot analysis was performed using the same antibody.

    Immunohistochemical analysis of paraffin-embedded mouse lung using E-Cadherin (24E10) Rabbit mAb.

    Western blot analysis of extracts from A204, SKMEL5, and NIH/3T3 cells using Slug (C19G7) Rabbit mAb.

    Confocal immunofluorescent analysis of A204 cells (left) and PANC-1 cells (right) using Slug (C19G7) Rabbit mAb (green). Actin filaments have been labeled with DY554 phalloidin (red).

    Western blot analysis of extracts from various cell lines using Snail (C15D3) Rabbit mAb.

    Western blot analysis of extracts from COS cells, mock transfected or transfected with a construct expressing human TCF8/ZEB1, using TCF8/ZEB1 (D80D3) Rabbit mAb.

    Western blot analysis of extracts from Jurkat, HT1080 and A172 cells using TCF8/ZEB1 (D80D3) Rabbit mAb.

    Flow cytometric analysis of HeLa cells (blue) and MCF7 cells (green) using E-Cadherin (24E10) Rabbit mAb.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma using Vimentin (D21H3) XP® Rabbit mAb.

    Immunohistochemical analysis of paraffin-embedded human tonsil using Vimentin (D21H3) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

    Western blot analysis of extracts from various cell lines using Vimentin (D21H3) XP® Rabbit mAb.

    Western blot analysis of extracts from various cell lines using Vimentin (D21H3) XP® Rabbit mAb #5741.

    Western blot analysis of extracts from various cell lines using E-Cadherin (24E10) Rabbit mAb #3195.

    Western blot analysis of extracts from various cell lines using N-Cadherin Antibody #4061.

    Western blot analysis of extracts from A-431 and ZR-75 cells using Claudin-1 Antibody #4933.

    Western blot analysis of extracts from various cell lines using Snail (C15D3) Rabbit mAb #3879.

    Western blot analysis of extracts from A-204, SK-MEL-5, and NIH/3T3 cells using Slug (C19G7) Rabbit mAb #9585.

    Western blot analysis of extracts from Jurkat, HT-1080 and A172 cells using TCF8/ZEB1 (D80D3) Rabbit mAb #3396.

    Flow cytometric analysis of HeLa cells, using Vimentin (D21H3) XP® Rabbit mAb (blue) compared to Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (red).

    Confocal immunofluorescent analysis of SNB19 cells using Vimentin (D21H3) Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

    Immunohistochemical analysis of paraffin-embedded mouse colon using Vimentin (D21H3) XP® Rabbit mAb.

    Western blot analysis of extracts from various cell lines using ZO-1 (D7D12) Rabbit mAb.

    Immunoprecipitation and western blot analysis of extracts from Hep G2 cells using ZO-1 (D7D12) Rabbit mAb. Lane 1 is 10% input.

    Confocal immunofluorescent analysis of HeLa (left) and NCI-H28 (right) cells using β-Catenin (D10A8) XP® Rabbit mAb (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

    Flow cytometric analysis of NCI-H28 (blue) or HeLa (green) cells using β-Catenin (D10A8) XP® Rabbit mAb.

    Immunohistochemical analysis of paraffin-embedded mouse colon using β-Catenin (D10A8) XP® Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

    Immunohistochemical analysis of paraffin-embedded cell pellets, HeLa (left) or NCI-H28 (right), using β-Catenin (D10A8) XP® Rabbit mAb.

    Chromatin immunoprecipitations were performed with cross-linked chromatin from 4 x 106 HCT 116 cells and either 20 μl of β-Catenin (D10A8) XP® Rabbit mAb or 2 μl of Normal Rabbit IgG #2729 using SimpleChIP® Enzymatic Chromatin IP Kit (Magnetic Beads) #9003. The enriched DNA was quantified by real-time PCR using SimpleChIP® Human Axin2 Intron 1 Primers #8973, SimpleChIP® Human CaMK2D Intron 3 Primers #5111, human c-Myc promoter primers, and SimpleChIP® Human α Satellite Repeat Primers #4486. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

    Confocal immunofluorescent analysis of mouse colon using β-Catenin (D10A8) XP® Rabbit mAb (green). Actin filaments were labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma using β-Catenin (D10A8) XP® Rabbit mAb.

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma using β-Catenin (D10A8) XP® Rabbit mAb.

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma using β-Catenin (D10A8) XP® Rabbit mAb.

    Western blot analysis of extracts from various cell lines using β-Catenin (D10A8) XP® Rabbit mAb #8480.

    Western blot analysis of extracts from various cell lines using ZO-1 (D7D12) Rabbit mAb #8193.

    Western blot analysis of extracts from A431 and MCF7 cells using Claudin-1 (D5H1D) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

    Immunoprecipitation of claudin-1 from A-431 cell extracts using Rabbit (DA1E) mAb IgG XP® Isotype Control #3900 (lane 2) or Claudin-1 (D5H1D) XP® Rabbit mAb (lane 3). Lane 1 is 10% input. Western blot was performed using Claudin-1 (D5H1D) Rabbit mAb.

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma using Claudin-1 (D5H1D) XP® Rabbit mAb.

    Immunohistochemical analysis of paraffin-embedded cell pellets, A-431 (left) and MCF7 (right), using Claudin-1 (D5H1D) XP® Rabbit mAb.

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma using Claudin-1 (D5H1D) XP® Rabbit mAb.

    Immunohistochemical analysis of paraffin-embedded human skin using Claudin-1 (D5H1D) XP® Rabbit mAb.

    Confocal immunofluorescent analysis of A172 (positive, left) and MCF7 (negative, right) cells using N-Cadherin (D4R1H) XP® Rabbit mAb (green). Blue pseudocolor= DRAQ5® #4084 (fluorescent DNA dye).

    Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma using N-Cadherin (D4R1H) XP® Rabbit mAb.

    Immunohistochemical analysis of paraffin-embedded A172 (positive, left) and MCF7 (negative, right) cell pellets using N-Cadherin (D4R1H) XP® Rabbit mAb.

    Immunohistochemical analysis of paraffin-embedded human colon using N-Cadherin (D4R1H) XP® Rabbit mAb. Note staining of myenteric plexus.

    Western blot analysis of extracts from A172 and MCF7 cells using N-Cadherin (D4R1H) XP® Rabbit mAb (upper) or β-Actin (D6A8) Rabbit mAb #8457 (lower).

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    货号：
    9782T
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