ER-Tracker Green (BODIPY FL Glibenclamide)

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Product Usage Information

ER-Tracker Green (BODIPY FL Glibenclamide) is supplied as a single 100 μg lyophilized vial. To make a 1 mM stock solution, reconstitute the solid in 128 μl of high quality DMSO. Dilute 1:500 directly into normal growth media for a working concentration of 2 μM and analyze immediately. Cells must be imaged live; DO NOT FIX! To reduce background fluorescence of the media, phenol red free media can be substituted for normal media prior to imaging.

应用

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背景

ER-Tracker Green (BODIPY FL Glibenclamide) is a cell-permeant, live cell stain that is highly selective for the endoplasmic reticulum (ER). This stain consists of the green-fluorescent BODIPY FL dye and glibenclamide. Glibenclamide (glyburide) binds to sulphonylurea receptors, the regulatory subunit of ATP-sensitive K+ channels prominently expressed in the ER. The pharmacological activity of glibenclamide could potentially affect ER function. Variable expression of sulphonylurea receptors in some specialized cell types may result in non-ER labeling.

翻译后修饰

unmodified

制备和贮存

保存方式

Store lyophilized solid or solution at –20°C dessicated and protected from light. Once reconstituted in DMSO, the solution should be aliquoted to avoid freeze-thaw cycles. If stored properly, this reagent is stable for 6 months.

参考图片

Confocal immunofluorescent analysis of live HeLa cells using ER-Tracker Green (BODIPY FL Glibenclamide) (green). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

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货号：

8787S

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询价

100ug

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