Rabbit anti-ErbB2 Recombinant Monoclonal Antibody(069-57)

WB result of ErbB2 Rabbit mAb Primary antibody: ErbB2 Rabbit mAb at 1/50000 dilution Lane 1: MCF7 whle cell lysate 5 µg Lane 2: SK-BR-3 whole cell lysate 5 µg Low expression control: MCF7 whole cell lysate Secondary antibody: #abs20040 at 1/10000 dilution Predicted MW: 185 kDa Observed MW: 185 kDa Exposure time: 30s

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-ErbB2 antibody was used at 1/1000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human breast cancer. Anti-ErbB2 antibody was used at 1/1000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows negative staining in paraffin-embedded human breast. Anti-ErbB2 antibody was used at 1/1000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.