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产品介绍
产品介绍
产品信息
商品描述
5-Ethynyl-uridine (EU) is a commonly used molecular biology reagent for RNA synthesis studies.
EU is readily taken up by living cells and phosphorylated through the pyrimidine salvage pathway. Generated 5-ethynyluridine-5’-triphosphate is incorporated by RNA polymerases I, II, and III into de novo RNA instead of uridine, but not into DNA.
EU-labeled nascent cellular RNA can be detected quickly and with high sensitivity via click chemistry following fluorescent visualization. Alkyne group attached at the 5-position of uridine in modified RNA reacts with dye or biotin azides via Cu(I)-catalyzed azide-alkyne cycloaddition (CuAAC).
Labeled RNA can be detected with different methods, e.g. fluorescent microscopy or flow cytometry, which allows estimating transcriptional levels in the cells.
分子量
268.22
可溶性
good in water, DMSO, DMF
外观
off white solid
组合货号
Ambient
背景
别名
1-((2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-ethynylpyrimidine-2,4(1H,3H)-dione
分子式
C11H12N2O6
CAS号
69075-42-9
制备和贮存
保存方式
Storage: 24 months after receival at -20°C in the dark. Transportation: at room temperature for up to 3 weeks.
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